PCR Hero
Sharaxaada Kit
Alaabtan waxaa ka mid ah Foregene jiilka cusub ee gaarka ah ee Taq——Foregene Taq Hero, dNTPs la hagaajiyay, MgCl2, falcelinta falcelinta, xoojisay falcelinta PCR, hagaajiye iyo xasiliye.Halyeyga 2× PCRTMNidaamka isku dhafka ah wuxuu leeyahay dulqaad sare oo ka hortagga PCR marka loo eego nidaamka isku-dhafka PCR ee caadiga ah, wuxuuna si fudud ula qabsan karaa PCR-weyneynta qaabab kala duwan oo adag.Nidaamka falcelinta gaarka ah iyo waxtarka sare ee Taq Hero ayaa ka dhigaya falcelinta PCR inay yeelato hufnaan sare u qaadis, gaar ahaan iyo dareen.
Tilmaamaha
Kit Taxanaha
PH-01013: 2× PCR HalyeyTMIsku qas 1.7ml ×3
PH-01014: 2× PCR HalyeyTMIsku qas 1.7ml ×6
PH-01015: 2× PCR HalyeyTMIsku qas 1.7ml ×24
Qaybaha kitka
2× PCR HalyeygaTMIsku darka waxaa ka mid ah: 0.1U Taq Hero/μl, 400μM dNTPs, 20mM Tris-HCl
Tilmaamaha & faa'iidooyinka
-Kalsoonida sare:6jeer ka mid ah Taq enzyme caadiga ah;
- Si degdeg ah axawaaraha kordhinta:xawaaruhu waa 5-10sec/kb, kaas oo 6-12 jeer ka badan kuwa caadiga ah ee Taq;
-Laqabsiga template oo badan: waxay si wax ku ool ah u kordhin kartaa noocyo kala duwan oo DNA ah oo kakan oo leh maadada GC sare oo ay adagtahay in la kordhiyo;
- Ka sarreeyaHufnaanta kordhinta: tirada wareegyada cod-weyneyntu way ka hooseeyaan ta enzyme Taq caadiga ah;
-Ska xoog badan Dulqaadka deegaanka: lagu meeleeyaa 37°C muddo todobaad ah, iyadoo la ilaalinayo in ka badan90%dhaqdhaqaaqa;
-Waxay leedahay 5'→3' dhaqdhaqaaqa DNA polymerase iyo 5'→3' dhaqdhaqaaqa exonuclease, iyada oo aan lahayn 3'→5' firfircoonida exonuclease.
Codsiga qalabka
- Kordhinta PCR ee jajabyada DNA-da
- Calaamadinta DNA-da
- isku xigxiga DNA
- PCR iyo dabada A
Kaydinta iyo nolosha shelf
Waxaa lagu kaydin karaa waqti dheer at -20 ° C;Isticmaalka joogtada ah, waxa lagu kaydin karaa 4°C muddo gaaban.