Shiinaha General Plasmid Mini Kit General Plasmid DNA Soo saarista Miniprep Mini Kits Soo saaraha iyo Iibiyaha |Foregene

Xirmooyinka Yaryar ee Plasmid-ka Guud ee Plasmid-ka Soo saaridda Miniprep Mini Kits

Xirmooyinka Yaryar ee Guud ee Plasmid-ka Guud ee Plasmid-ka Soo saarista Miniprep-yar ee Xirmooyinka Sawirka leh

Sharaxaada Qalabka:

Si dhakhso leh uga nadiifi DNA-da plasmid ee tayada sare leh bakteeriyada la beddelay ee tijaabooyinka bayoolojiga joogtada ah sida isbeddelka iyo dheefshiidka ensaymka.

◮Ka saar RNA adigoon ku darin RNase

◮Ku habboon-centrifugation-ka waxaa lagu sameeyaa heerkulka qolka

◮Degdeg ah-qaliinka waxa lagu dhamayn karaa 20 daqiiqo

◮Ammaan- lama isticmaalo reagent organic

◮daahirnimo sare-OD260/280≈1.7-1.9

Email noogu soo dir U soo deji sidii PDF

Faahfaahinta Alaabta

Tags Product

Sharaxaada Kit

Soo saarista aashitada nucleic-ka ah ee FOREGENE iyo alaabooyinka taxanaha ah ee sifaynta ayaa laga soo saari karaa oo lagu sifeyn karaa asiidhyo nuclei ah oo tayo sare leh oo laga keenay ilo kala duwan iyo muunado ka kooban qaybo kakan.Alaabooyinka taxanaha soo saarista DNA waxay ku salaysan yihiin DNA-Kaliya tiirarka nadiifinta xuubka silica iyo reagents qaacido gaar ah oo ka nadiifin kara DNA-da tayada sare leh noocyo kala duwan.

Qalabka Guud ee Plasmid Mini Kit wuxuu isticmaalaa tignoolajiyada tiirka nadiifinta DNA-Kaliya iyo qaacido lysis SDS oo hufan si looga helo DNA plasmid oo tayo sare leh bakteeriyada 20 daqiiqo gudahood.Xuubabka silica-kaliya ee DNA-da ee loo isticmaalo tiirarka laf-dhabarka waa shay cusub oo u gaar ah FOREGENE, kaas oo si hufan u xajin kara DNA-da, kana saari kara RNA, borotiinnada wasakhaysan, ion iyo xeryahooda kale ee organic ee unugyada ilaa xadka ugu badan.Awoodda ugu badan ee isku xidhka DNA-da plasmid ee tiirka nadiifinta DNA-kaliya waa 60μg DNA.Qadarka DNA-da plasmid dhab ahaantii la soosaaray waxay la xiriirtaa mugga xalka bakteeriyada, tirada nuqullada plasmid-ka (nuqul sare, nuqul hoose), noocyada, xaaladaha dhaqanka iyo arrimo kale.

Tilmaamaha

Qalabka Guud ee Plasmid Mini
Waxa ku jira Kit	DE-01001	DE-01002	DE-01003
Waxa ku jira Kit	50T	100T	250T
Buffer S1	10ml	20ml	50ml
Buffer S2	10ml	20ml	50ml
Buffer S3	25ml	50ml	125ml
Buffer PW	25ml	50ml	125ml
Buffer WB2	15ml	30ml	75ml
Buffer EB	10ml	20ml	50ml
Tiirka-Kaliya DNA	50	100	250
Buugga	1	1	1

Tilmaamaha & faa'iidooyinka

- Ma jirto faddarayn RNase: Tiirka DNA-kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in laga saaro RNA ee ku jira DNA-da plasmidka iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo laga fogaanayo faddaraynta shaybaarka ee RNase dibadda ah.

Xawaaraha degdega ah: Soo saarista DNA-da Plasmid waxaa lagu dhamayn karaa 20 daqiiqo gudahood.

- Badbaadsan: Soo saarista reageent organic looma baahna.

- Tayada sare: Plasmid-ka lagu helo soosaarku ma laha RNA, malaha RNase iyo maadada ion aad u hooseeya, kaas oo buuxin kara shuruudaha tijaabooyinka kala duwan.

Codsiga qalabka

Soo saarista DNA-da Plasmid iyo nadiifinta bakteeriyada Gram-negative.

Socodka shaqada

Jaantuska

Kaydinta iyo nolosha shelf

Qalabkan waxa lagu kaydin karaa 12 bilood xaaladaha qalalan ee heerkulka qolka (15-25 ℃);Haddii loo baahdo in la kaydiyo wakhti dheer, waxa lagu kaydin karaa 2-8 ℃.

Fiiro gaar ah: Haddii lagu kaydiyo heerkul hooseeya, xalku wuxuu u nugul yahay roobab.Kahor intaan la isticmaalin, hubso inaad geliso xalka qalabka heerkulka qolka muddo wakhti ah.Haddii loo baahdo, ku sii kuleyli biyaha qubeyska 37 ℃ 10 daqiiqo si aad u milmaan roobabka, oo isku qas ka hor isticmaalka.

Hore: Qalabka Go'doominta DNA-ga Dhirta Xirmooyinka Nadaafadda DNA-da ee Genomic Plant Protocol

Xiga: Caleenta dhirta Tooska ah PCR iyo xirmo-UNG (la'aanteed Qalabka Muunalada) borotokoolka tooska ah PCR ee agabka dhirta

Halkan ku qor fariintaada oo noo soo dir