1kb Salaanka DNA
Sharaxaada Qalabka:
◮Jaranjarada DNA-da 1Kb waxa ay ka kooban tahay 13 qaybood oo DNA ah oo labanlaab ah, xoojinta labanlaabmay ee 1kb iyo 5kb waxa ay caawinaysaa in la kala saaro qaybaha kala duwan.Ka hooseeya 1kb, saddex qaybood oo ah 250bp, 500bp, iyo 750bp ayaa lagu daray, cabbirka jajabka gaaban ayaa qiyaas ahaan loo xisaabin karaa iyadoo lagu saleynayo tan.Isku-duubnaanta afarta qaybood ee 6kb, 7kb, 8kb, iyo 10kb waa kala badh, taas oo faa'iido u leh si loo helo saameyn kala-goyn wanaagsan.
Sharaxaada
Jaranjarada DNA-da 1Kb waxa ay ka kooban tahay 13 qaybood oo DNA ah oo labanlaab ah, xoojinta labanlaabmay ee 1kb iyo 5kb waxa ay caawinaysaa in la kala saaro qaybaha kala duwan.Ka hooseeya 1kb, saddex qaybood oo ah 250bp, 500bp, iyo 750bp ayaa lagu daray, cabbirka jajabka gaaban ayaa qiyaas ahaan loo xisaabin karaa iyadoo lagu saleynayo tan.Isku-duubnaanta afarta qaybood ee 6kb, 7kb, 8kb, iyo 10kb waa kala badh, taas oo faa'iido u leh si loo helo saameyn kala-goyn wanaagsan.
Alaabtani waxay hore uga koobnayd 1x Loading Buffer.Waxaad u qaadan kartaa 2-6μl si toos ah electrophoresis iyadoo loo eegayo baahida tijaabada.Sawirka electrophoresis waa cad, ku habboon oo wax ku ool ah.
13ka xabo ee alaabtan min yar ilaa wayn waa: 250bp, 500bp, 750bp, 1kb, 1.5kb, 2kb, 3kb, 4kb, 5kb, 6kb, 7kb, 8kb, 10kb.Haddii mugga loading yahay 5μl, 1kb iyo 5kb band waa 100ng;6kb, 7kb, 8kb, iyo 10kb band waa 25ng;inta soo hartayna waa 50ng.
Tilmaamaha
| DL-30011 | 50 Diyaarin (250ml) |
| DL-30012 | 200 Diyaarin (1ml) |
Qaybaha kitka
Macluumaadka dareeraha kaydinta
10mM Tris-HCl (pH 8.4) 10mM EDTA
0.02% BPB
5% glycerin
Xoog saarista alaabta
Isku-duubnaanta alaabadu waxay ku saabsan tahay 130μg/ml
Electroferogram
Xaaladaha kaydinta
Fadlan ku kaydi 4℃(fadlan ku kaydi -20℃kaydinta muddada dheer)
