RT-PCR Easyᵀᴹ I (Hal Tallaabo)
Sharaxaada Qalabka:
◮Xirmada hal-tallaabo ah waxay awood u siinaysaa qoraal-celinta iyo PCR in lagu sameeyo isla tuubo isku mid ah.Waxa kaliya oo ay u baahan tahay in lagu daro template RNA, asal PCR gaar ah iyo ddH-RNase-free2O.
◮Falanqaynta tirada waqtiga-dhabta ah ee RNA si dhakhso ah oo sax ah ayaa loo samayn karaa.
◮Qalabku waxa uu isticmaalaa reagent transcription u gaar ah Foregene iyo Foregene HotStar Taq DNA Polymerase oo ay weheliso nidaam falcelin gaar ah si si wax ku ool ah loo wanaajiyo waxtarka xoojinta iyo gaar ahaaneed ee falcelinta.
◮Habka falcelinta ee la wanaajiyey ayaa ka dhigaysa fal-celintu inay yeelato dareen-helid sare, degganaansho kulayleed oo xooggan, iyo dulqaad wanaagsan.
Tilmaamaha
Foregene Hal-Tallaabo RT-PCR FududTM Alaabooyinka taxanaha ah waxay xaqiiqsadaan falcelinta isku dhafan ee RNA ilaa DNA-da laba-geesoodka ah, taas oo ah, qoraal-celinta iyo kordhinta PCR waxaa lagu dhammeeyaa isla tuubada centrifuge-ka falcelinta iyo isla habka falcelinta, taas oo fududaynaysa tillaabooyinka tijaabada., wanaajisaatwuxuu barnaamij tijaabo ah, iyohagaajinaysaa tijaabada hufnaan.
RT-PCR FududTM I(Hal Tilaabo) waa xirmo hal-tallaabo ah oo RT-PCR ah oo ay si gaar ah u soo saartay Foregene, kaas oo ogaanaya hawlgal joogto ah oo ka bilaabma RT ilaa PCR isla tuubo isku mid ah.Qalliinku waa mid fudud oo degdeg ah, kaas oo si wax ku ool ah u yareeyn kara wasakhda inta lagu jiro tijaabada.2×RT-PCR FududTM Isku-darku wuxuu leeyahay dulqaad xooggan, xasilloonida kulaylka, waxtarka sare iyo gaar ahaaneedity for kordhin.
Qaybaha kitka
| RT-PCR FududTMI (Hal Talaabo) | ||
| Qalabka ku jira | RT-02011 | RT-02012 |
| 100T (Nidaamka 20μl) | 500T (Nidaamka 20μl) | |
| 2× RT-PCR FududTM Isku qas | 1ml | 1.7ml×3 |
| RNase-free ddH2O | 1.7ml | 1.7ml×3 |
| Buugga tilmaamaha | 1 gabal | 1 gabal |
Tilmaamaha & faa'iidooyinka
- Xidhmada hal-tallaabo ah waxay awood u siinaysaa qoraal-ku-noqoshada iyo PCR in lagu sameeyo isla tuubo isku mid ah.Waxa kaliya oo ay u baahan tahay in lagu daro template RNA, asal PCR gaar ah iyo ddH-RNase-free2O.
- Falanqaynta tirada waqtiga-dhabta ah ee RNA si degdeg ah oo sax ah ayaa loo samayn karaa.
- Xidhmadani waxay isticmaashaa reagent transcription u gaar ah Foregene iyo Foregene HotStar Taq DNA Polymerase oo ay weheliso nidaam falcelin gaar ah si ay si wax ku ool ah u wanaajiso waxtarka xoojinta iyo gaar ahaaneed ee falcelinta.
-Nidaamka falcelinta ee la wanaajiyey ayaa ka dhigaysa fal-celintu inay yeelato dareen ogaansho sare leh, degganaansho kulayleed oo xoog badan, iyo dulqaad wanaagsan.
Socodka shaqada
Kaydinta iyo nolosha shelf
1.Xaaladaha gaadiidka
Geedi socodka oo dhan ee sanduuqa barafka heerkulka hooseeya, si loo hubiyo in qalabku ku jiro xaalad <4 °C.
2. Xaaladaha kaydinta
Qalabka waxaa lagu kaydiyaa heerkulka -20 ° C.Ku kaydi alaabta qaboojiyaha heerkulka joogtada ah -20 ° C isla markiiba ka dib markii la helo.Haddii shuruudaha kaydinta ay habboon yihiin,talaabtu ma hoos u dhigi doonto wax qabad kasta inta lagu jiro muddada ansaxinta 1-sano.
