Qalabka Nadiifinta PCR Nidaamka Nadiifinta PCR ee DNA-da saafiga ah
Sharaxaada Qalabka:
Si dhakhso leh u nadiifi oo u hel jajabyada DNA-da tayada sare leh ee nidaamka PCR;Haddii aad rabto inaad kala tagto oo aad hesho jajab DNA oo gaar ah, fadlan dooro xirmada soo kabashada jel.
◮ Soo kabashada DNA-da oo ballaadhan: Qaybaha DNA-da oo gaaban sida 60bp iyo inta 10kb ah waa la soo ceshan karaa.
◮ Waxtarka soo kabashada sareWaxtarka soo kabashada guud ahaan waa in ka badan 80%, kan ugu sarreeyana wuxuu gaari karaa in ka badan 95%.
Xawaaraha dheereeya:Fudud in la shaqeeyo, soo kabashada jajabka DNA-da waxaa lagu dhameyn karaa 15 daqiiqo gudahood iyo dimer-ka si toos ah ayaa loo saari karaa iyada oo aan dib u soo kabashada jel.
◮ SafeefSoo saarista reagent organic looma baahna.
◮ Tayo sare: Qaybaha DNA-da ee la helay waa nadiif sare, kuwaas oo la kulmi kara tijaabooyin kala duwan oo xiga.
Sharaxaada
Xirmadani waxay isticmaashaa tiirka lafdhabarta iyo caanaha ay samaysay shirkadeena, kaas oo si hufan uga soo kaban kara jajabyada DNA-da ee nadiifka ah ee nidaamka PCR.Tiirka sifaynta ayaa si hufan u xidhi kara DNA-da, waxaana la jaanqaadi kara reagent qaacido gaar ah oo si toos ah meesha uga saaraya dimer-yaraha iyada oo aan jel soo kaban.
Qalabku wuxuu soo kabanayaa jajabyo badan oo DNA ah.Xaaladaha caadiga ah, jajabyada DNA ee gaaban sida 60bp waa la soo celin karaa.Ugu yaraan 30 μl oo eluent ah ayaa loo isticmaali karaa si loo kordhiyo uruurinta DNA-da la soo kabsaday.
Qalabka waa sahlan tahay in lagu shaqeeyo wuxuuna leeyahay talaabooyin yar.Waxay u baahan tahay oo kaliya dhowr sentifugation si ay u baaraan shaybaaro badan isku mar, iyo daahir sare oo jajabka DNA soo kabsaday waxaa lagu heli karaa 15 daqiiqo gudahood.
Tilmaamaha
50 Diyaarin, 100 Diyaarin, 250 Diyaarin
Qaybaha kitka
| Buffer BD* |
| Buffer BD-S |
| Buffer WB1 |
| Buffer EB |
| Tiirka-Kaliya DNA |
| Tilmaamaha |
Tilmaamaha & faa'iidooyinka
Soo-kabashada DNA-da oo ballaadhan: jajabyada DNA-da oo gaaban sida 60bp iyo inta u badan 10kb waa la soo ceshan karaa.
Waxtarka soo kabashada sare: waxtarka soo kabashada guud ahaan waa in ka badan 80%, kan ugu sarreeyana wuxuu gaari karaa in ka badan 95%.
Xawaaraha degdega ah: fududahay in la shaqeeyo, soo kabashada jajabka DNA-da waxaa lagu dhameyn karaa 15 daqiiqo gudahood iyo dimer-ka si toos ah ayaa loo saari karaa iyada oo aan dib u soo kabashada gel.
-Badbaadada: Soo saarista reagents organic looma baahna.
-Tayada sare: Qaybaha DNA-da ee la helay waa nadiif sare, kuwaas oo la kulmi kara tijaabooyin kala duwan oo xiga.
Codsiga qalabka
Xirmadani waxay ku habboon tahay dib u soo celinta jajabyada DNA-da (60bp-10kb) ee alaabta PCR.
Socodka shaqada
Kaydinta iyo nolosha shelf
Qalabkan waxaa lagu kaydin karaa 12 bilood xaaladaha qalalan ee heerkulka qolka (15-25 ° C);Haddii loo baahdo in lagu kaydiyo wakhti dheer, waxa lagu kaydin karaa 2-8 ° C.
Fiiro gaar ah: Haddii lagu kaydiyo heerkul hooseeya, xalku wuxuu u nugul yahay roobab.Kahor intaan la isticmaalin, hubso inaad geliso xalka qalabka heerkulka qolka muddo wakhti ah.Haddii loo baahdo, ku sii kululee biyaha qubeyska 37°C 10 daqiiqo si aad u milanto daadka, oo ku qas kahor isticmaalka.
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