Qalabka SuperDirectᵀᴹ PCR Kit(UNG) -EDTA Dhiigga Tooska ah ee PCR Master Mix for Genotyping of Blood
Tilmaamaha
200 × 20μl rxns, 2000 × 20μl rxns
2× Aad u FududTMMix(UNG) -EDTA: Iyada oo ku saleysan 2×SuperEasyTMMix-EDTA, dUTP waxa loo isticmaalaa halkii dTTP, iyo UNG ensaymes oo hoos u dhigi kara template ka kooban dUTP la isku mar lagu daray, kaas oo si wax ku ool ah uga hortagi kara wasakheynta alaabta PCR weynaynta.Ka kooban yahay D-Taq DNA Polymerase, dNTPs, MgCl2, falcelinta falcelinta, xoojisay falcelinta PCR, hagaajinta iyo xasiliyaha, iwm. Falcelinta PCR, kaliya ku dar dhiiga oo dhan ee xinjirowga lidka ku ah, alwaaxyada, iyo ddH2O ilaa 2× SuperEasy u dhigmaTMMix(UNG) -EDTA si loogu isticmaalo falcelinta PCR
6× DNA Loading Buffer :Qaybta kaydinta kuma jiraan SDS.Waxaa lagu talinayaa in la isticmaalo 6 × DNA Loading Buffer oo ay ku jiraan xirmada marka la samaynayo agarose gel electrophoresis si loo helo natiijooyin elektrophoresis wanaagsan.Ha isticmaalin Loading Buffer ka kooban SDS, haddii kale waxaa jiri doona dabo weyn oo iftiin dhalaalaya haadka inta lagu jiro electrophoresis, taas oo saameyn doonta natiijooyinka tijaabada.
Qaybaha kitka
2× Aad u FududTMIsku qas(UNG)-EDTA |
6× DNA Loading Buffer |
Tilmaamaha |
Tilmaamaha & faa'iidooyinka
- Ma jirto baahi loo qabo nadiifinta DNA-da waqti-qaadashada iyo qaaliga ah, la'aan daweyn, iyo isticmaalka tooska ah ee dhiigga oo dhan sida template PCR.
-Nidaamku waxa uu leeyahay awood xoog wayn oo xoog leh waxana uu si xasaasi ah u ogaan karaa genome-ka iyo jajabyada DNA-da ee ajnabiga ah ee dhiigga ku jira.
-2× SuperEasyTMMix(UNG)-EDTA waxay leedahay dulqaad dhiig oo sarreeya: ilaa 45% dhiiga bini'aadamka iyo 20% ee dhiiga jiirka.
-Muunada si buuxda ayaa loo xidhay, markaa looma baahna in laga welwelo wasakhaynta muunadda iyo natiijooyinka PCR beenta ah.Nidaamka PCR-ka-hortagga wasakheynta 2×SuperEasyTMMix(UNG)-EDTA, waxay si wax ku ool ah u tirtirtaa wasakhowga ay keenaan alaabta PCR, waxayna hubisaa gaar ahaan iyo saxnaanta cod-weyneysiinta.
Codsiga qalabka
U heellan in si toos ah loo aqoonsado PCR ee EDTA dhiigga dhan ee xinjirowga lidka ku ah.( oo ay ku jiraan: dhiigga dadka, dhiigga jiirka, dhiigga digaagga, dhiigga shimbiraha, dhiigga lo'da, dhiigga eyga, iwm.)
Socodka shaqada
Kaydinta iyo nolosha shelf
Qalabkan waxa lagu kaydiyaa -20°C.
2× Aad u FududTMMix(UNG)-EDTA, Haddii si joogta ah loo isticmaalo, waxa kale oo lagu kaydin karaa 4°C kaydinta muddada gaaban (la isticmaalo 10 maalmood gudahood).
6× DNA Loading Buffer, Waxa lagu kaydin karaa at 4°C ama -20°C muddo dheer.