Blood SuperDirectᵀᴹ PCR Kit-Heparin Dhiiga Tooska ah ee PCR Master Mix for Genotyping of Blood
Sharaxaada Qalabka:
Si toos ah u isticmaal heparin xinjirowga lidka ku ah dhiigga oo dhan tusaale ahaan u-qaadista PCR, oo leh dareen sare iyo hufnaanta sare-u-qaadista.
◮Looma baahna nadiifinta DNA-da waqti-qaadashada iyo qaaliga ah, daawayn la'aan, iyo isticmaalka tooska ah ee dhiiga oo dhan qaab PCR.
◮Nidaamku wuxuu leeyahay awood xoog-weyneyn wuxuuna si xasaasi ah u ogaan karaa genome-ka iyo jajabyada DNA-da ee ajnabiga ah ee dhiigga ku jira.
◮2× Aad u FududTMIsku-dhafka Heparin Dulqaadka Dhiigga Sare: ilaa 45% ee dhiiga bini'aadamka iyo 20% ee dhiiga jiirka.
◮Muunadda si buuxda ayaa loo xidhay, markaa looma baahna in laga welwelo wasakhowga muunadda iyo natiijooyinka PCR ee beenta ah.
Tilmaamaha
200 × 20μl rxns, 2000 × 20μl rxns
2× Aad u FududTMIsku-dhafka Heparin: Waxa ku jira D-Taq DNA Polymerase, dNTPs, MgCl2, falcelinta falcelinta PCR, kor u qaadida falcelinta, hagaajinta, iwm2O ilaa 2× SuperEasy u dhigmaTMMix-Heparin oo loo isticmaalo falcelinta PCR.
6× DNA Loading Buffer ：Qaybta kaydinta kuma jiraan SDS.Waxaa lagu talinayaa in la isticmaalo 6 × DNA Loading Buffer oo ay ku jiraan xirmada marka la samaynayo agarose gel electrophoresis si loo helo natiijooyin elektrophoresis wanaagsan.Ha isticmaalin Loading Buffer ka kooban SDS, haddii kale waxaa jiri doona dabo weyn oo iftiin dhalaalaya haadka inta lagu jiro electrophoresis, taas oo saameyn doonta natiijooyinka tijaabada.
Qaybaha kitka
| 2× SuperEasyTM Mix-Heparin |
| 6× DNA Loading Buffer |
| Tilmaamaha |
Tilmaamaha & faa'iidooyinka
- Looma baahna nadiifinta DNA-da waqti-qaadashada iyo qaaliga ah, daawayn la'aan, iyo isticmaalka tooska ah ee dhiiga oo dhan qaab PCR.
-Nidaamku waxa uu leeyahay awood xoog wayn oo xoog leh waxana uu si xasaasi ah u ogaan karaa genome-ka iyo jajabyada DNA-da ee ajnabiga ah ee dhiigga ku jira.
-2× SuperEasyTMIsku-dhafka Heparin Dulqaadka Dhiigga Sare: ilaa 45% ee dhiiga bini'aadamka iyo 20% ee dhiiga jiirka.
-Muunada si buuxda ayaa loo xidhay, markaa looma baahna in laga welwelo wasakhaynta muunadda iyo natiijooyinka PCR beenta ah.
Codsiga qalabka
U heellan si toos ah aqoonsiga PCR ee Heparin ka-hortagga xinjirowga dhiigga oo dhan.( oo ay ku jiraan: dhiigga dadka, dhiigga jiirka, dhiigga digaagga, dhiigga shimbiraha, dhiigga lo'da, dhiigga eyga, iwm.)
Socodka shaqada
Kaydinta iyo nolosha shelf
Xirmadan waa in lagu kaydiyaa -20℃.
2× Aad u FududTMMix-Heparin, Haddii si joogta ah loo isticmaalo, waxaa lagu kaydin karaa 4 ° C kaydinta muddada gaaban (la isticmaalo 10 maalmood gudahood).
6× DNA Loading Buffer, Waxa lagu kaydin karaa at 4°C ama -20°C muddo dheer.
