Xirmada I/II ee Tooska ah ee Iilka Dhirta (la'aanteed Aalado Muunado)
Sharaxaada Qalabka:
◮Ma jiro nadiifinta DNA-da waqti-qaadashada iyo qaaliga ah (Waqtiga, shaqada iyo kharashka la badbaadiyay!)
◮Wax yar
◮Fudud - muunada waxaa lagu soo rogi karaa falcelinta PCR ama falcelinta lysis iyada oo aan la jarin ama la shiidi
◮2 X Isku darka — yaraynta khaladaadka rarka muunada iyo wakhtiga diyaarinta nidaamka falcelinta
◮Diyaarinta qaab-dhismeed degdeg ah ayaa lagu dhammeyn karaa 10 daqiiqo, falcelinta PCR waxaa lagu dhameyn karaa 50 daqiiqo
◮Wax soo saarka sare — falcelinta lysis waxaa lagu dhammayn karaa saxan PCR 96-wanaagsan
Sharaxaada
Alaabtani waxay isticmaashaa nidaam u gaar ah si ay u liso miraha dhirta.lysate-ka waxaa loo isticmaali karaa qaab-dhismeedka iyada oo aan la nadiifin.Ka dib marka lagu daro aasaasayaasha, PCR Mix ee xirmadan ayaa loo isticmaali karaa xoojinta.
Tilmaamaha
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Qaybaha kitka
| Abuurka Dhirta ee tooska ah ee PCR Kit I | |
| Qaybta I | Buffer SP1 |
| Buffer SP2 | |
| 6×DNA Loading Buffer | |
| Qaybta II | 2× Seed PCR FududTMIsku qas |
| Abuurka dhirta tooska ah PCR Kit II | |
| Qaybta I | Buffer SP1 |
| Buffer SP2 | |
| 6×DNA Loading Buffer | |
| Qaybta II | 2× Seed PCR FududTMIsku qas |
Tilmaamaha & faa'iidooyinka
■ Ma jiro waqti-qaadasho iyo nadiifin DNA-da qaali ah
■ Waxyaabo yar
■ Fudud-muunada waxaa lagu soo rogi karaa falcelinta PCR ama falcelinta lysis iyada oo aan la jarin ama la shiidi karin
■ 2X Isku qas-yaraynta khaladaadka rarka muunada iyo wakhtiga diyaarinta nidaamka falcelinta
■ Degdeg ah-diyaarinta template waxaa lagu dhamayn karaa 10 daqiiqo, falcelinta PCR waxaa lagu dhamayn karaa 50 daqiiqo
■ Wax soo saarka sare-falcelinta lysis waxa lagu dhamaystiri karaa saxan PCR 96-wanaagsan.
Xuduudaha Kit
Codsiga: Aqoonsiga dhirta transgenic, genotyping, iwm.
Tusaalaha: Ku beer abuurka dhirta ama unugyo leh polysaccharide hoose iyo ka kooban polyphenol.
Cabbirka Tusaalaha: Hal abuur oo dhan, 1-100mg unug dhirta
Kala duwanaanshaha ogaanshaha: alaabta PCR ≤1kb
Socodka shaqada
Electroferogram
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