Abuurka Dhirta (xajmi weyn iyo mid dhexe) Qalabka PCR Plus ee Tooska ah II-UNG (aan lahayn Qalabka Muunalada)
Sharaxaada
Alaabtani waxay isticmaashaa nidaam falcelin lysis gaar ah, kaas oo si dhakhso ah u sii deyn kara DNA-ga genomic ee shaybaarada abuurka dhirta leh polysaccharides sare iyo polyphenols (sida muuska, suufka, iwm.) si loogu isticmaalo falcelinta PCR, sidaas darteed waxay si gaar ah ugu habboon tahay baaritaanka hidde-socodka ballaaran.Si loo daboolo baahiyaha tijaabooyinka ogaanshaha kala duwan, xirmadani waxay adeegsan kartaa noocyo badan oo muunado ah (sida iniinaha oo dhan, goynta unugyada yaryar, ama muunadaha dhulka ee abuur badan)Waxaa ka mid ah, muunadaha abuurka ee weli u baahan in la biqlo, waxaad dooran kartaa inaad gooyso unugyada unugyada yaryar (1-5mg) oo aan ahayn embriyaha abuurka ee tijaabooyinka;Tijaabooyin u baahan muunad iyo tijaabin tiro badan oo muunado ah, waxaad dooran kartaa inaad isku qasto muunado badan oo aad isku darsato.Ka dib marka la shiido qaybo leh dhexroor qiyaastii ah 0.5mm, tijaabada waa la samayn karaa (ugu yaraan 0.1% muunada bartilmaameedka ayaa la ogaan karaa).
Tilmaamaha
50T,200T,500T,2000T
Qaybaha kitka
Qalab Dhirta Tooska ah ee PCR Plus Kit II-UNG Abuurka dhirta polyphenol ee polysaccharide (xajmi weyn iyo mid dhexe) xirmada tooska ah ee PCR-UNG | |||||
Halabuurka Kit | TP-0318T | TP-03181 | TP-03182 | TP-03183 | |
50 T | 200 T | 500 T | 2000 T | ||
Qaybta I | Buffer SSP1 | 24ml | 96ml | 120ml×2 | 480ml×2 |
Buffer SP2 | 3ml | 10ml | 25ml | 100ml | |
6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
Qaybta II | Buffer SSP2 | 8ml | 32ml | 80ml | 80ml×4 |
2× Seed PCR EasyTM Mix | 500ml | 1ml×2 | 1.7ml×3 | 1.7ml×12 | |
Buugga | 1 | 1 | 1 | 1 |
Tilmaamaha iyo faa'iidooyinka
- Ma jiro waqti-qaadasho iyo nadiifin DNA qaali ah.
- Baahida muunadku way yar tahay, kaliya qaado hal abuur.
- Looma baahna daawayn gaar ah sida shiidi iyo burburin, qalliinkuna waa sahlan yahay.
-Nidaamka PCR ee la hagaajiyay waxa uu awood u siinayaa PCR in uu yeesho gaar gaar ah iyo dulqaad xoogan.
- Nidaamka PCR-ka-hortagga wasakheynta 2× Seed PCR EasyTM Isku qas (UNG), kaas oo si wax ku ool ah u baabi'in kara wasakhowga ay keenaan alaabta PCR oo hubisa gaar ahaan iyo saxnaanta cod-weyneysiinta.
Socodka shaqada
Xaaladaha kaydinta
1.Gaadiidka
Geedi socodka oo dhan ee sanduuqa barafka heerkulka hooseeya, si loo hubiyo in xidhmada Qaybta II ay ku jirto xaalad <4 ℃ ah.
2.Kaydinta
Qaybta I ee xirmadan waa in lagu kaydiyaa heerkulka qolka ama 2-8°C.
Qaybta II waa in lagu kaydiyaa -20°C.