Shiine Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish) Soo saaraha iyo Alaabta |Foregene

Kalluunka Zebra Kalluunka tooska ah ee PCR Kit-UNG Tooska PCR Lysis Reagent(zebrafish)

Qalabka Zebra Kalluunka Tooska ah ee PCR Kit-UNG Tooska PCR Lysis Reagent(zebrafish) Sawirka

Sharaxaada Qalabka:

Isticmaalka tooska ah ee lysate zebrafish sida template for amplification PCR, ma soosaarid gooni ah DNA, degdeg ah iyo dareen sare.Waxaa lagu daray UNG nidaamka ka-hortagga wasakheynta si meesha looga saaro waxyaabaha beenta ah.

◮Looma baahna nadiifin waqti-qaadasho iyo qaali ah.

◮Dalabka muunadku wuu yar yahay, kaliya qaado hal abuur.

◮Looma baahna daaweyn gaar ah sida shiidi iyo burburin, qalliinkuna waa sahlan yahay.

◮Nidaamka PCR ee la hagaajiyay wuxuu awood u siinayaa PCR inuu yeesho gaar gaar ah iyo dulqaad adag oo loogu talagalay horjoogayaasha falcelinta PCR.

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Faahfaahinta Alaabta

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Sharaxaada

Xirmadani waxay isticmaashaa hab-dhis u gaar ah si ay si dhakhsa ah u sii deyso DNA-da genomic ee zebrafish iyo unugyada kale ee kalluunka biyaha saafiga ah, dabada dabada ama shaybaarka ukunta kalluunka ee falcelinta PCR, marka waxay si gaar ah ugu habboon tahay baaritaanka hidde-sidaha baaxadda leh.Habka looga sii daayo DNA-da genomic ee kaydiyaha lysis waxa lagu dhammeeyaa 10-30 daqiiqo gudahood 65°C.Looma baahna habab kale sida borotiinka iyo ka saarista RNA, DNA-da raadraaca ee la sii daayayna waxaa loo isticmaali karaa qaab-dhismeedka falcelinta PCR.

2× PCR EasyTM Mix (UNG) waxay isticmaashaa dUTP halkii ay ka isticmaali lahayd dTTP iyada oo ku saleysan 2× PCR EasyTM Mix, waxayna ku daraysaa UNG enzyme (Uracil-N-glycosylase) taas oo hoos u dhigi karta template ka kooban dUTP isku mar.Kahor falcelinta PCR-ga, ensaymka UNG waxaa loo isticmaalaa hoos u dhigista alaabta PCR ee ay ku jirto uracil.Enzyme UNG wax saameyn ah kuma yeelan doono qaab-dhismeedka aan ku jirin uracil, si loo hubiyo gaarnimada iyo saxnaanta xoojinta iyo ka hortagga suurtagalnimada baaritaanka hidda-socodka ballaaran.Wasakhaynta alaabta PCR ayaa dhacday.

D-Taq DNA polymerase waa DNA polymerase si gaar ah u soo saaray Foregene ee falcelinta PCR tooska ah.D-Taq DNA polymerase waxay leedahay dulqaad xoog leh oo loogu talagalay xakameynta falcelinta PCR ee kala duwan, waxayna si wax ku ool ah u kordhin kartaa tirada DNA-da ee hababka falcelinta kakan ee kala duwan, iyo xawaaruhu wuxuu gaari karaa 2Kb/min, kaas oo si gaar ah ugu habboon falcelinta PCR tooska ah.

Tilmaamaha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Qaybaha kitka

Qaybta I	Buffer FP
	Kahortagga Protease
	6× DNA Loading Buffer
Qaybta II	2× PCR Fudud^TMIsku qas(UNG)
Tilmaamaha

Tilmaamaha & faa'iidooyinka

■ Looma baahna nadiifin waqti badan iyo qaali ah.

■ Baahida muunada waa mid yar, in yar oo 1mg kalluun caudal fin ah ama 10 ukun oo kalluun ah ayaa la tijaabin karaa.

■ Looma baahna daawayn gaar ah sida shiidi iyo burburin, qaliinkuna waa sahlan yahay.

■ Nidaamka PCR-ka-hortagga wasakheynta 2× PCR EasyTM Mix (UNG), kaas oo si wax ku ool ah u baabi'in kara wasakhowga ay keenaan alaabta PCR oo hubisa gaar ahaan iyo saxnaanta cod-weyneeynta.

■ Nidaamka PCR ee la hagaajiyay wuxuu ka dhigayaa PCR inay yeeshaan gaar gaar ah oo adag iyo dulqaad xooggan ee ka hortagga falcelinta PCR.

Xuduudaha Kit

Codsiga: Kalluunka Zebra iyo kalluunka kale ee biyaha macaan.

DNA-da laga sii daayay muunada lysis: kaliya waxaa loo adeegsaday qaab PCR.

Qalabka waxaa loo isticmaali karaa ujeedooyinka soo socda: aqoonsiga transgenosis, genotyping xayawaanka, iwm.

Socodka shaqada

Kaydinta iyo nolosha shelf

Qaybta I ee xirmadan waa in lagu kaydiyaa 2-8℃.

Reagent Buffer FP waxa lagu kaydin karaa 12 bilood xaaladaha qalalan;waxa lagu kaydin karaa 2-8℃ kaydinta dheer

v Reagent Foregene Protease wuxuu leeyahay qaacido gaar ah.Si loo hubiyo dhaqdhaqaaqeeda iyo xasiloonideeda, fadlan ku kaydi at 4℃ ilaa 12 bilood.

vReagent 6×DNA Loading Buffer waxa lagu kaydin karaa 4℃ ama -20℃ wakhti dheer.

Qaybta II ee xirmadan waa in lagu kaydiyaa -20℃.

vReagent 2×PCR EasyTM Mix(UNG), haddii si joogto ah loo isticmaalo, waxa kale oo lagu kaydin karaa 4℃ kaydinta muddada gaaban (isticmaal 10 maalmood gudahood).

Hore: Qalabka Zebra Kalluunka tooska ah ee PCR-ga tooska ah ee PCR Lysis Reagent

Xiga: Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Tail)(loogu talagalay Genotyping)

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