China Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Tail)(oo loogu talagalay Genotyping) Soo saaraha iyo Iibiyaha |Foregene

Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Tail)(loogu talagalay Genotyping)

Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Tail)(oo loogu talagalay Genotyping) Sawirka

Sharaxaada Qalabka:

Si toos ah u isticmaal jiirka dabada lysate qaab tusaale ah oo loogu talagalay kordhinta PCR, iyada oo aan la soo saarin DNA gaar ah, degdeg iyo dareen sare.

◮Looma baahna nadiifin waqti-qaadasho iyo qaali ah.

◮Dalabka muunadku wuu yar yahay, kaliya qaado hal abuur.

◮Looma baahna daaweyn gaar ah sida shiidi iyo burburin, qalliinkuna waa sahlan yahay.

◮Nidaamka PCR ee la hagaajiyay wuxuu awood u siinayaa PCR inuu yeesho gaar gaar ah iyo dulqaad adag oo loogu talagalay horjoogayaasha falcelinta PCR.

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Faahfaahinta Alaabta

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Tilmaamaha

Xirmadani waxay isticmaashaa nidaam u gaar ah lysis-ka si ay si dhakhso ah uga sii daayaan DNA-da genomic ee dabada jiirka iyo muunadaha dhegta jiirka ee falcelinta PCR, sidaa darteed waxay si gaar ah ugu habboon tahay baaritaanka hidde-sidaha baaxadda leh.

Habka looga sii daayo DNA-da genomic ee ka soo baxa lysis-ka waa la dhammeeyey10-30 daqiiqo gudahoodatheerkulka qolka (20-25°C).Kuleyl looma baahna, hababka kale sida borotiinka iyo ka saarista RNA looma baahna.Qaddarka raadadka DNA-da ee la sii daayay waxaa loo isticmaali karaa qaab-celin falcelin PCR ah.

2×M-PCR Fudud^TMIsku-darku waxa uu dulqaad xooggan u leeyahay hor-joogayaasha falcelinta PCR, waxayna isticmaali kartaa lysate-ka muunadda si loogu tijaabiyo qaab-dhismeedka si hufan oo gaar ah.Dib-u-kicinta waxay ka kooban tahay Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, celiyaha falcelinta, PCR optimizer iyo stabilizer.

D-Taq DNA polymerase waa DNA polymerase si gaar ah u soo saaray Foregene ee falcelinta PCR tooska ah.D-Taq DNA polymerase waxay leedahay dulqaad xoog leh oo loogu talagalay xakameynta falcelinta PCR ee kala duwan, waxayna si wax ku ool ah u kordhin kartaa tirada DNA-da ee hababka falcelinta kakan ee kala duwan, iyo xawaaruhu wuxuu gaari karaa 2Kb/min, kaas oo si gaar ah ugu habboon falcelinta PCR tooska ah.

Tilmaamaha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Qaybaha kitka

Qaybta I	Buffer NP
	Foregene Protease Plus I
	6× DNA Loading Buffer
Qaybta II	2× M PCR EasyTM Mix
Tilmaamaha

Tilmaamaha & faa'iidooyinka

■Looma baahna nadiifin waqti-qaadasho iyo qaali ah.

■ Baahida muunada waa yar tahay, kaliya qaado dabada jiirka (ama dhagaha jiirka ee dhexroorkoodu yahay 2-5mm) oo ka sarreeya 2mm.

■ Fudud in lagu shaqeeyo, waa la nadiifin karaa iyada oo aan kuleyl lahayn si loo damiyo protease-ka, iyo sheyga la shiiday waxaa si toos ah loogu qaadi karaa falcelinta PCR.

■ Foregene Mouse Tail Genomic DNA Kit (DE-05211) ayaa loo isticmaali karaa in laga soo saaro DNA-da genomic dabada jiirka ee la warshadeeyay.

■ Nidaamka PCR ee la hagaajiyay wuxuu ka dhigayaa PCR inay yeeshaan gaar gaar ah oo adag iyo dulqaad xooggan ee ka hortagga falcelinta PCR.

Xuduudaha Kit

Codsiga: dabada jiirka, dhegaha jiirka, muruqa iyo unugyo kale.

DNA-da laga sii daayay muunada lysis: kaliya waxaa loo adeegsaday qaab PCR.

Qalabka waxaa loo isticmaali karaa ujeedooyinka soo socda: aqoonsiga transgenosis, genotyping xayawaanka, iwm.

Socodka shaqada

Kaydinta iyo nolosha shelf

Qaybta I ee xirmadan waa in lagu kaydiyaa 2-8℃.

Reagent Buffer FP waxa lagu kaydin karaa 12 bilood xaaladaha qalalan;waxa lagu kaydin karaa 2-8℃ kaydinta dheer

Reagent Foregene Protease wuxuu leeyahay qaacido gaar ah.Si loo hubiyo dhaqdhaqaaqeeda iyo xasiloonideeda, fadlan ku kaydi at 4℃ ilaa 12 bilood.

Reagent 6×DNA Loading Buffer waxa lagu kaydin karaa 4℃ ama -20℃ wakhti dheer.

Qaybta II ee xirmadan waa in lagu kaydiyaa -20℃.

Reagent 2×PCR EasyTM Mix, haddii si joogto ah loo isticmaalo, waxa kale oo lagu kaydin karaa 4℃ kaydinta muddada gaaban (isticmaal 10 maalmood gudahood).

Hore: Kalluunka Zebra Kalluunka tooska ah ee PCR Kit-UNG Tooska PCR Lysis Reagent(zebrafish)

Xiga: Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Mouse Tail)(loogu talagalay Genotyping)

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