With this motto in mind, we've turn into one of quite possibly the most technologically innovative, cost-efficient, and price-competitive saarays for factory Outlets for China High Efficiency PCR, Taq Plus DNA PolymeraseTani waxay awood u siinaysaa inay isticmaalaan xasilooni maskaxeed oo kalsooni leh.
Anigoo maskaxda ku hayna hal-ku-dheggan, waxaan u beddelnay mid ka mid ah kuwa ugu macquulsan ee ugu casrisan tignoolajiyada, kharash-ku-ool ah, iyo soo-saareyaasha qiimaha-ku-tartanka badanKordhinta PCR ee Shiinaha, Qpcr, Xirfadda, daacadnimada had iyo jeer waa aasaas u ah himiladeena.Waxaan had iyo jeer la jaanqaadnay u adeegida macaamiisha, abuurista ujeedooyinka maaraynta qiimaha iyo u hoggaansanaanta daacadnimada, daacadnimada, fikradda maaraynta joogtada ah.

Tilmaamaha

50×20μl rxns,200×20μl rxns,1000×20μl rxns,2000×20μl rxns

2X Real PCR EasyTM Mix-Taqman oo ay bixiso xirmada Real Time PCR EasyTM-Taqman waa nidaam horudhac ah oo cusub kaas oo isticmaala baaritaanno fluorescent gaar ah oo loogu talagalay falcelinta kor u qaadida PCR ee waqtiga-dhabta ah, taas oo si weyn u wanaajin karta gaar ahaan badeecada iyo dareenka falcelinta.ROX waxa loo bixiyaa sidii dheeha xakamaynta gudaha.

2X Real PCR EasyTM Mix-Taqman waxa ku jira bilowga kulul ee gaarka ah ee Foregene Taq DNA Polymerase.Marka la barbar dhigo enzymes-ka caadiga ah ee Taq, waxay leedahay faa'iidooyinka hufnaanta sare u qaadida, awood xoog leh oo gaar ah oo xoog leh iyo heerar is-gaarsiineed oo hooseeya.Waxay yarayn kartaa kordhinta aan gaarka ahayn waxayna hagaajin kartaa saxnaanta PCR.

Qaybaha kitka

Tilmaamaha & faa'iidooyinka

■ Fudud-2X PCR Isku-dhafan si loo yareeyo khaladka tijaabada iyo wakhtiga hawlgalka

■ Bakhaar gaar ah oo la hagaajiyay iyo kuleelka bilawga ah ee Taq enzyme waxay ka hortagi kartaa kordhinta aan gaarka ahayn iyo samaynta dimer-ka

■ Dareenka sare-wuxuu ogaan karaa nuqullo hoose oo template ah

■ Kala duwanaansho wanaagsan - oo ku habboon inta badan qalabka PCR ee tirada dhabta ah

Codsiga qalabka

falanqaynta qPCR

Socodka Shaqada

Garaaf

Kaydinta iyo nolosha shelf

Xirmadan waa in lagu kaydiyaa meel ka durugsan iftiinka waana in lagu kaydiyaa -20℃.Haddii si joogto ah loo isticmaalo, waxa kale oo lagu kaydin karaa at 4 ℃ muddo gaaban (10 maalmood) .Iyadoo halkudheg this maskaxda ku hay, waxaan u soo jeesannay mid ka mid ah ilaa xad ugu suurto gal ah innovative teknoolajiyada, kharashka-ku-ool ah, iyo saarayaasha qiimaha-tartan for Warshadda China High Efficiency PCR, Taq Plus DNA Polymerase # P10032 tayo sare leh, si ay u bilaabaan our tayo leh tayo sare leh, At 2. lagu sameeyay Japan, laga bilaabo soo iibinta agabka ilaa habaynta.Tani waxay awood u siinaysaa inay isticmaalaan xasilooni maskaxeed oo kalsooni leh.
Goobaha warshadeed eeKordhinta PCR ee Shiinaha, Qpcr, Xirfadda, daacadnimada had iyo jeer waa aasaas u ah himiladeena.Waxaan had iyo jeer la jaanqaadnay u adeegida macaamiisha, abuurista ujeedooyinka maaraynta qiimaha iyo u hoggaansanaanta daacadnimada, daacadnimada, fikradda maaraynta joogtada ah.

Wax calaamado kordhin ah ma jiraan

1.Taq DNA Polymerase ee qalabka ku jira waxa uu lumiyaa dhaqdhaqaaqiisa sababtoo ah kaydinta aan habboonayn ama dhacaysa xirmada.
Talo soo jeedin: Xaqiiji xaaladaha kaydinta qalabka;dib ugu dar qaddarka saxda ah ee Taq DNA Polymerase nidaamka PCR ama iibso Xirmada PCR ee wakhtiga-dhabta ah ee tijaabooyinka la xidhiidha.

2.Waxaa jira inhibitors badan oo Taq DNA Polymerase ku jira template DNA.
Talo-soo-jeedin: Dib-u-nadiifi qaab-dhismeedka ama yaree tirada qaab-dhismeedka la isticmaalay.

3.Mg2 + xooga saaridu kuma haboona.
Talo soo jeedin: Xoojinta Mg2+ ee 2× Real PCR Mix ee aanu bixino waa 3.5mM.Si kastaba ha ahaatee, qaar ka mid ah aasaasayaasha gaarka ah iyo qaab-dhismeedka, fiirsashada Mg2+ ayaa laga yaabaa inay sareeyso.Sidaa darteed, waxaad si toos ah ugu dari kartaa MgCl2 si aad u wanaajiso fiirsashada Mg2+.Waxaa lagu talinayaa in la kordhiyo Mg2+ 0.5mM mar kasta si loo hagaajiyo.

4.Xaaladaha kor u qaadida PCR kuma haboona, isku xigxiga ama xooga saaridu waa khalad.
Talo-soo-jeedin: xaqiiji sax ahaanshiyaha isku xigxiga asaasiga ah iyo furaha oo aan hoos loo dhigin;Haddii calaamada-weyneyntu aysan fiicneyn, isku day inaad hoos u dhigto heerkulka soo jiidashada oo aad si habboon u hagaajiso diiradda aasaasiga ah.

5.Qaddarka template waa mid aad u yar ama aad u badan.
Talo-soo-jeedin: Samee qaab-dhismeed toosan oo dheellitiran, oo dooro fiirsashada template-ka oo leh saamaynta PCR ee ugu fiican ee tijaabada PCR-da ee Waqtiga-dhabta ah.

NTC waxay leedahay qiime aad u sarreeya

1.Wasakhowga reagent ee uu keeno inta lagu jiro hawlgalka.
Talo-soo-jeedin: Ku beddel reageyaal cusub oo loogu talagalay tijaabooyinka PCR ee waqtiga-dhabta ah.

2. faddarayn ayaa dhacday intii lagu jiray diyaarinta nidaamka falcelinta PCR.
Talo soo jeedin: Qaado tallaabooyinka ilaalinta lagama maarmaanka ah inta lagu jiro hawlgalka, sida: xidhashada galoofyada cinjirka ah, adigoo isticmaalaya caarada pipette leh filtar, iwm.

3.Qalabka hore ayaa hoos u dhacay, iyo hoos u dhac ku yimaadda aasaasayaasha ayaa sababi doona cod-weyneeyn aan gaar ahayn.
Talo soo jeedin: Isticmaal SDS-PAGE electrophoresis si aad u ogaatid in furayaasha ay hoos u dhaceen iyo in kale oo ku beddel qalab cusub oo loogu talagalay tijaabooyinka PCR ee wakhtiga dhabta ah.

Dimer Primer ama kordhin aan gaar ahayn

1.Xoojinta Mg2+ kuma habboona.
Talo-soo-jeedin: Xoojinta Mg2+ ee 2× Real PCR EasyTM Mix ee aanu bixino waa 3.5mM.Si kastaba ha ahaatee, qaar ka mid ah aasaasayaasha gaarka ah iyo qaab-dhismeedka, fiirsashada Mg2+ ayaa laga yaabaa inay sareeyso.Sidaa darteed, waxaad si toos ah ugu dari kartaa MgCl2 si aad u wanaajiso fiirsashada Mg2+.Waxaa lagu talinayaa in la kordhiyo Mg2+ 0.5mM mar kasta si loo hagaajiyo.

2. Heerkulka PCR ee annealing ayaa aad u hooseeya.
Talo soo jeedin: Kordhi heerkulka PCR-da ee nuglaanta 1℃ ama 2℃ wakhti kasta.

3.Alaabada PCR waa mid aad u dheer.
Talo soo jeedin: Dhererka alaabta PCR ee wakhtiga-dhabta ah waa in ay ahaataa inta u dhaxaysa 100-150bp, oo aan ka badnayn 500bp.

4.Qalabka hore ayaa hoos u dhacay, iyo hoos u dhigista muraayadaha waxay u horseedi doontaa muuqaalka kor u qaadista gaarka ah.
Talo soo jeedin: Isticmaal SDS-PAGE electrophoresis si aad u ogaatid in furayaasha ay hoos u dhaceen iyo in kale oo ku beddel qalab cusub oo loogu talagalay tijaabooyinka PCR ee wakhtiga dhabta ah.

5.Nidaamka PCR waa mid aan haboonayn, ama nidaamku aad buu u yar yahay.
Talo soo jeedin: Nidaamka falcelinta PCR-ga oo aad u yar ayaa sababi doonta in saxnaanta ogaanshaha ay yaraato.Way fiicantahay in la isticmaalo habka falcelinta ee uu ku taliyay qalabka PCR ee tirada badan si dib loogu socodsiiyo tijaabada PCR-da ee wakhtiga dhabta ah.

Ku celcelinta liidata ee qiimayaasha tirada

1.Qalabka waa cilad.
Soo jeedin: Waxaa laga yaabaa inay jiraan khaladaad u dhexeeya dalool kasta oo PCR ah ee qalabka, taasoo keentay dib u soo saarid xumo inta lagu jiro maaraynta heerkulka ama ogaanshaha.Fadlan hubi sida waafaqsan tilmaamaha qalabka u dhigma.

2. Nadiifinta muunada ma wanaagsana.
Talo-soo-jeedin: Tijaabooyin aan nadiif ahayn waxay u horseedi doonaan dib-u-soo-saar liidata tijaabada, oo ay ku jirto nadiifinta qaab-dhismeedka iyo aasaasayaasha.Way fiicantahay in la nadiifiyo qaab-dhismeedka, iyo aasaasayaasha waxaa sida ugu fiican u nadiifiya SDS-BOGG.

3.The nidaamka PCR diyaarinta iyo wakhtiga kaydinta waa mid aad u dheer.
Talo soo jeedin: U isticmaal nidaamka PCR ee wakhtiga dhabta ah tijaabada PCR isla markaaba diyaarinta ka dib, oo ha kaga tagin meel dheer.

4.Xaaladaha kor u qaadida PCR kuma haboona, isku xigxiga ama xooga saaridu waa khalad.
Talo-soo-jeedin: xaqiiji sax ahaanshiyaha isku xigxiga asaasiga ah iyo furaha oo aan hoos loo dhigin;Haddii calaamada-weyneyntu aysan fiicneyn, isku day inaad hoos u dhigto heerkulka soo jiidashada oo aad si habboon u hagaajiso diiradda aasaasiga ah.

5.Nidaamka PCR waa mid aan haboonayn, ama nidaamku aad buu u yar yahay.
Talo soo jeedin: Nidaamka falcelinta PCR-ga oo aad u yar ayaa sababi doonta in saxnaanta ogaanshaha ay yaraato.Way fiicantahay in la isticmaalo habka falcelinta ee uu ku taliyay qalabka PCR ee tirada badan si dib loogu socodsiiyo tijaabada PCR-da ee wakhtiga dhabta ah.