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Horudhac HS Taq DNA Polymerase

Sawirka sifaysan ee HS Taq DNA Polymerase Foreasy
  • Horudhac HS Taq DNA Polymerase

Sharaxaada Qalabka:

◮Qeexitaan sare: Insaymiska oo leh firfircooni bilow-kuleyl sare leh.

◮Kordhinta degdega ah: 10 ilbiriqsi/kb.

◮La qabsiga qaab-dhismeedka sare: waxaa loo isticmaali karaa in si hufan loo kordhiyo HighGCqiimahaiyonoocyo kala duwan oo ay adag tahay in la kordhiyo template DNA.

◮Daacadnimo adag: Aaminnimadu waa 6 jeerof Enzyme Taq caadiga ah.

xoog hor leh


Faahfaahinta Alaabta

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FAQ

Sharaxaada

Foreasy HS Taq DNA Polymerase waa enzim cusub oo Taq ah oo lagu muujiyey bakteeriyada Escherichia coli ee injineernimada farsamada dib-u-habaynta hidda-wadaha.Ka dib markii enzyme lagu daaweeyaa hab gaar ah, waxa's dhaq-dhaqaaqa waa la hakiyaa ka hor firfircoonida kulaylka, taas oo ka hortagaysa kordhinta aan gaarka ahayn ee ay sababtay ciribtirka aan gaarka ahayn ee furayaasha ama dimers-yada hoos yimaada xaaladaha heerkulka hooseeya.Alaabtani waxay ku habboon tahay PCR React aadka u gaarka ahion, M ultiple x PCR , ka kooban GC sare (> 60%),lehqaabdhismeedka sareama mid kalegenom asalka xoogganicsxoojinta iyo genom-ga weynicsogaanshaha kordhinta.Insaymku wuxuu leeyahay 5' → 3' firfircoonida polymerase DNA iyo 5' → 3' firfircoonida exonuclease, laakiin maya 3' → 5' dhaqdhaqaaqa exonuclease.

Qaybaha kitka

Qayb IM-01021 IM-01022 IM-01023
Horudhac HS Taq DNA Polymerase (5 U/μL)  5000 U (1 ml)  50 KU (10 ml)  500 KU (100 ml)
2× Taq Reaction Buffer  25ml ×5  250 ml ×5  500 ml × 25

Tilmaamaha & faa'iidooyinka

- Si gaar ah u sarreeya: Insaymiska oo leh hawl-bilow heer sare ah.

- Kordhinta degdega ah: 10 ilbiriqsi/kb.

- La qabsiga qaab-dhismeedka sare: waxaa loo isticmaali karaa in si hufan loo kordhiyo HighGCqiimahaiyonoocyo kala duwan oo ay adag tahay in la kordhiyo template DNA.

- Daacadnimo adag: Aaminnimadu waa 6 jeerof Enzyme Taq caadiga ah.

Codsiga qalabka

- Nidaam PCR/qPCR kala duwan iyo nidaamka PCR tooska ah

- jajab DNA oo la xoojiyay PCR

- sumadda DNA

- Isku-dabarid DNA

- PCR iyo dabada A

Qeexida Hawsha

1U : Qadarka enzyme loo baahan yahay in lagu daro 10 nmol oo ahDNAgalay walxo aashito ah oo aan milmi karin iyadoo la isticmaalayo shahwada salmon firfircoon ee DNA sida template / primer, 74 °C, 30 daqiiqo.

Xaaladda falcelinta

Heerkulka Waqtiga Falcelinta Waqtiga wareegga
37°C 5 daqiiqo 1
94°C 5 daqiiqo 1
94°C 10 ilbiriqsi  40
60°C 10 ilbiriqsi

Fiiro gaar ah:Nidaamyada 10 µL iyo 20 µL, ku dar mug siman oo saliid macdan ah haddii wareegga kulaylku aanu lahayn dabool kulaylka.

Xaaladaha falcelinta PCR way kala duwan yihiin iyadoo ku xiran xaaladaha qaabdhismeedka qaab-dhismeedka, furayaasha, iyo wixii la mid ah.Hawlgalka gaarka ah, waxaa lagama maarmaan ah in la naqshadeeyo xaaladaha falcelinta ugu fiican, oo ay ku jiraan heerkulka nuugista, wakhtiga kordhinta, iwm., marka loo eego shuruudaha gaarka ah sida nooca template, cabbirka jajabka bartilmaameedka, isku xigxiga saldhigga ee jajabka la xoojiyay, iyo nuxurka GC iyo dhererka asaasiga ah.

Kaydinta

-20 ± 5 °C 2 sano ama at -80 °C kaydinta muddada-dheer.

  • Hore:
  • Xiga:

    • Wax calaamado kordhin ah ma jiraan

    1.Taq DNA Polymerase ee qalabka ku jira waxa uu lumiyaa dhaqdhaqaaqiisa sababtoo ah kaydinta aan habboonayn ama dhacaysa xirmada.
    Talo soo jeedin: Xaqiiji xaaladaha kaydinta qalabka;dib ugu dar qaddarka saxda ah ee Taq DNA Polymerase nidaamka PCR ama iibso Xirmada PCR ee wakhtiga-dhabta ah ee tijaabooyinka la xidhiidha.

    2.Waxaa jira inhibitors badan oo Taq DNA Polymerase ku jira template DNA.
    Talo-soo-jeedin: Dib-u-nadiifi qaab-dhismeedka ama yaree tirada qaab-dhismeedka la isticmaalay.

    3.Mg2 + xooga saaridu kuma haboona.
    Talo soo jeedin: Xoojinta Mg2+ ee 2× Real PCR Mix ee aanu bixino waa 3.5mM.Si kastaba ha ahaatee, qaar ka mid ah aasaasayaasha gaarka ah iyo qaab-dhismeedka, fiirsashada Mg2+ ayaa laga yaabaa inay sareeyso.Sidaa darteed, waxaad si toos ah ugu dari kartaa MgCl2 si aad u wanaajiso fiirsashada Mg2+.Waxaa lagu talinayaa in la kordhiyo Mg2+ 0.5mM mar kasta si loo hagaajiyo.

    4.Xaaladaha kor u qaadida PCR kuma haboona, isku xigxiga ama xooga saaridu waa khalad.
    Talo-soo-jeedin: xaqiiji sax ahaanshiyaha isku xigxiga asaasiga ah iyo furaha oo aan hoos loo dhigin;Haddii calaamada-weyneyntu aysan fiicneyn, isku day inaad hoos u dhigto heerkulka soo jiidashada oo aad si habboon u hagaajiso diiradda aasaasiga ah.

    5.Qaddarka template waa mid aad u yar ama aad u badan.
    Talo-soo-jeedin: Samee qaab-dhismeed toosan oo dheellitiran, oo dooro fiirsashada template-ka oo leh saamaynta PCR ee ugu fiican ee tijaabada PCR-da ee Waqtiga-dhabta ah.

    NTC waxay leedahay qiime aad u sarreeya

    1.Wasakhowga reagent ee uu keeno inta lagu jiro hawlgalka.
    Talo-soo-jeedin: Ku beddel reageyaal cusub oo loogu talagalay tijaabooyinka PCR ee waqtiga-dhabta ah.

    2. faddarayn ayaa dhacday intii lagu jiray diyaarinta nidaamka falcelinta PCR.
    Talo soo jeedin: Qaado tallaabooyinka ilaalinta lagama maarmaanka ah inta lagu jiro hawlgalka, sida: xidhashada galoofyada cinjirka ah, adigoo isticmaalaya caarada pipette leh filtar, iwm.

    3.Qalabka hore ayaa hoos u dhacay, iyo hoos u dhac ku yimaadda aasaasayaasha ayaa sababi doona cod-weyneeyn aan gaar ahayn.
    Talo soo jeedin: Isticmaal SDS-PAGE electrophoresis si aad u ogaatid in furayaasha ay hoos u dhaceen iyo in kale oo ku beddel qalab cusub oo loogu talagalay tijaabooyinka PCR ee wakhtiga dhabta ah.

    Dimer Primer ama kordhin aan gaar ahayn

    1.Xoojinta Mg2+ kuma habboona.
    Talo-soo-jeedin: Xoojinta Mg2+ ee 2× Real PCR EasyTM Mix ee aanu bixino waa 3.5mM.Si kastaba ha ahaatee, qaar ka mid ah aasaasayaasha gaarka ah iyo qaab-dhismeedka, fiirsashada Mg2+ ayaa laga yaabaa inay sareeyso.Sidaa darteed, waxaad si toos ah ugu dari kartaa MgCl2 si aad u wanaajiso fiirsashada Mg2+.Waxaa lagu talinayaa in la kordhiyo Mg2+ 0.5mM mar kasta si loo hagaajiyo.

    2. Heerkulka PCR ee annealing ayaa aad u hooseeya.
    Talo soo jeedin: Kordhi heerkulka PCR-da ee nuglaanta 1℃ ama 2℃ wakhti kasta.

    3.Alaabada PCR waa mid aad u dheer.
    Talo soo jeedin: Dhererka alaabta PCR ee wakhtiga-dhabta ah waa in ay ahaataa inta u dhaxaysa 100-150bp, oo aan ka badnayn 500bp.

    4.Qalabka hore ayaa hoos u dhacay, iyo hoos u dhigista muraayadaha waxay u horseedi doontaa muuqaalka kor u qaadista gaarka ah.
    Talo soo jeedin: Isticmaal SDS-PAGE electrophoresis si aad u ogaatid in furayaasha ay hoos u dhaceen iyo in kale oo ku beddel qalab cusub oo loogu talagalay tijaabooyinka PCR ee wakhtiga dhabta ah.

    5.Nidaamka PCR waa mid aan haboonayn, ama nidaamku aad buu u yar yahay.
    Talo soo jeedin: Nidaamka falcelinta PCR-ga oo aad u yar ayaa sababi doonta in saxnaanta ogaanshaha ay yaraato.Way fiicantahay in la isticmaalo habka falcelinta ee uu ku taliyay qalabka PCR ee tirada badan si dib loogu socodsiiyo tijaabada PCR-da ee wakhtiga dhabta ah.

    Ku celcelinta liidata ee qiimayaasha tirada

    1.Qalabka waa cilad.
    Soo jeedin: Waxaa laga yaabaa inay jiraan khaladaad u dhexeeya dalool kasta oo PCR ah ee qalabka, taasoo keentay dib u soo saarid xumo inta lagu jiro maaraynta heerkulka ama ogaanshaha.Fadlan hubi sida waafaqsan tilmaamaha qalabka u dhigma.

    2. Nadiifinta muunada ma wanaagsana.
    Talo-soo-jeedin: Tijaabooyin aan nadiif ahayn waxay u horseedi doonaan dib-u-soo-saar liidata tijaabada, oo ay ku jirto nadiifinta qaab-dhismeedka iyo aasaasayaasha.Way fiicantahay in la nadiifiyo qaab-dhismeedka, iyo aasaasayaasha waxaa sida ugu fiican u nadiifiya SDS-BOGG.

    3.The nidaamka PCR diyaarinta iyo wakhtiga kaydinta waa mid aad u dheer.
    Talo soo jeedin: U isticmaal nidaamka PCR ee wakhtiga dhabta ah tijaabada PCR isla markaaba diyaarinta ka dib, oo ha kaga tagin meel dheer.

    4.Xaaladaha kor u qaadida PCR kuma haboona, isku xigxiga ama xooga saaridu waa khalad.
    Talo-soo-jeedin: xaqiiji sax ahaanshiyaha isku xigxiga asaasiga ah iyo furaha oo aan hoos loo dhigin;Haddii calaamada-weyneyntu aysan fiicneyn, isku day inaad hoos u dhigto heerkulka soo jiidashada oo aad si habboon u hagaajiso diiradda aasaasiga ah.

    5.Nidaamka PCR waa mid aan haboonayn, ama nidaamku aad buu u yar yahay.
    Talo soo jeedin: Nidaamka falcelinta PCR-ga oo aad u yar ayaa sababi doonta in saxnaanta ogaanshaha ay yaraato.Way fiicantahay in la isticmaalo habka falcelinta ee uu ku taliyay qalabka PCR ee tirada badan si dib loogu socodsiiyo tijaabada PCR-da ee wakhtiga dhabta ah.

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