Wadarta Guud ee Qalabka Go'doonka RNA
Sharaxaada Qalabka:
Si wax ku ool ah uga saar DNA adoo isticmaalaya Tiirka Nadiifinta DNA
Ka saar DNA adigoon ku darin DNA-ga
Fudud - dhammaan hawlgallada waxaa lagu dhammeeyaa heerkulka qolka
Dhaqso-qalliinka waxa lagu dhamayn karaa 30 daqiiqo gudahood
Badbaadsan — lama isticmaalin reageent organic
Tilmaamaha
50 Diyaarin, 200 Diyaarin
Qalabku wuxuu isticmaalaa tiirka lafdhabarta iyo caanaha ay soo saartay Foregene, kaas oo si hufan u soo saari kara nadiif sare iyo wadarta tayada sare leh ee RNA ee unugyada dhirta kala duwan ee leh polysaccharides sare ama polyphenols.Waxay bixisaa tiirka DNA-Nadiifinta kaas oo si fudud uga saari kara DNA-da genomic ee sare iyo unugyada lysate.Tiirka RNA-kaliya ayaa si wax ku ool ah u xidhi kara RNA.Qalabku wuxuu farsamayn karaa tiro badan oo muunado isku mar ah.
Nidaamka oo dhan kuma jiro RNase, markaa RNA la safeeyey ma hoos u dhacayso.Buffer PRW1 iyo Buffer PRW2 waxay hubin karaan in RNA-ga la helay aanu wasakhayn borotiinka, DNA, ions, iyo xeryahooda organic.
Qaybaha Kit
| Buffer PSL1, Buffer PS, Buffer PSL2 |
| Buffer PRW1, Buffer PRW2 |
| RNase-free ddH2O, Tiirka Nadiifinta DNA |
| Tiirka RNA-Kaliya |
Tilmaamaha & faa'iidooyinka
■ Ku shaqaynta heerkulka qolka (15-25 ℃) inta lagu jiro habka oo dhan, iyada oo aan baraf lagu maydhin iyo heerkul hoose
■ Qalabka RNase-free oo dhameystiran, looma baahna in laga walwalo xaalufka RNA.
■ Si gaar ah ugu habboon sifaynta RNA ee muunada dhirta ee polysaccharides iyo polyphenols.
Tiirka Nadiifinta DNA-da wuxuu si gaar ah ugu xidhan yahay DNA-da, si uu qalabku uga saaro wasakhaynta DNA-da iyada oo aan lagu darin DNA-da.
∎ Wax-soo-saarka RNA sare: Tiirka-RNA-kaliya iyo caanaha gaarka ah ayaa si hufan u nadiifin kara RNA.
■ Xawaaraha dheereeya: fududahay in lagu shaqeeyo waxaana lagu dhameyn karaa 30 daqiiqo gudahood.
■ Badbaadada: looma baahna reageent organic.
■ Tayo sare leh: Qaybaha RNA ee la safeeyey waa nadiif sare, ka madhan borotiin iyo wasakh kale, waxayna la kulmi karaan codsiyo tijaaboyin oo kala duwan.
Xuduudaha alaabta
■ Codsiyada hoose: isku-dhafka cDNA-xarunta kowaad, RT-PCR, cloning molecular, Northern Blot, iwm.
■ Tusaale: Unugyada dhirta cusub ama barafaysan ee polysaccharides iyo polyphenols
■ Qiyaasta: 50mg unug dhirta
∎ Awoodda ugu badan ee RNA ee isku xidhka tiirka sifaynta: 80 μg
■ Mugga iftiinka: 50-200 μl
Codsiga qalabka
Waxay ku habboon tahay soo saarista iyo nadiifinta wadarta RNA ee muunada unugyada dhirta cusub ama barafaysan (gaar ahaan unugyada caleenta geedka cusub) oo leh polysaccharide sare iyo ka kooban polyphenol.
Socodka shaqada
Jaantuska
Plant Total RNA Go'doominta Qalabka Plus waxa uu farsameeyay 50mg oo caleemo cusub ah oo polysaccharides iyo polyphenols ah, iyo 5% RNA la safeeyey ayaa lagu tijaabiyay electrophoresis.
1: Mooska
2: Ginkgo
3: Cudbi
4: rummaanka
Kaydinta iyo nolosha shelf
Qalabkan waxaa lagu kaydin karaa 24 bilood xaaladaha qalalan ee heerkulka qolka (15-25 ℃);Haddii loo baahdo in la kaydiyo wakhti dheer, waxa lagu kaydin karaa 2-8 ℃.
Buffer PSL1 waxa la dhigi karaa 4℃ muddo 1 bilood ah kadib marka lagu daro β-mercaptoethanol (waxa lagu talinayaa in lagu daro isla wakhtiga tijaabada).
