Mouse Tail DNA Mini Kit Xirmooyinka Soo saarista DNA ee Genomic ee Dabada Mouse
Sharaxaada Qalabka:
Qalabka ugu dhaqsaha badan adduunka ee lagu soo saaro DNA-da dabada jiirka, kaas oo 1 saac gudaheed ka soo saari kara DNA-ga genomic-ga ah ee tayada sare leh.
◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.
◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.
◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.
◮Badbaadada:Looma baahna soo saarista reagent organic.
◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.
Sharaxaada
Sababtoo ah qaab dhismeedka iyo sifooyinka dabada jiirka lafteeda, soo saarista dabada jiirka DNA-da ayaa had iyo jeer dhibay cilmi-baarayaasha: mid waa in waqtiga farsamaynta dabada jiirka uu dheer yahay oo DNA-da genomic si fudud ayaa loo dhimayaa, kan kalena way adagtahay in la xakameeyo iyo nadiifinta DNA-da genomic ee la soo saaray ayaa hooseysa.
Xirmadani waxay isticmaashaa tiirka DNA-kaliya oo si gaar ah u xidhi kara DNA-da, nooc cusub oo Foregene Protease ah iyo nidaam u gaar ah, kaas oo dheefi kara muunadaha dabada jiirka 45 daqiiqo gudahood, taas oo hoos u dhigaysa hoos u dhaca DNA-da genomic iyo soo gaabinaysa wakhtiga samaynta muunadda.Xirmadu waxay soo saari kartaa 10-20μg oo tayo sare leh, DNA genomic saafi ah oo nadiif ah laga bilaabo 0.5-1cm dabada dhallinta ama qaangaarka dabada 60-90min.
Qalabka DNA-ka kaliya ee silica gel matrix ee loo isticmaalo tiirarka lafdhabarta ayaa ah shay cusub oo shirkadda ah, kaas oo si hufan oo gaar ah u xajin kara DNA-da.Awoodda adsorption ee ugu badan ee DNA waa 80μg.Nidaamka kaydinta gaarka ah iyo nidaamka sare u qaadida waxay sare u qaadi kartaa ka saarida RNA, borotiinnada wasakhaysan, ions iyo xeryahooda kale ee organic ee unugyada.Jajabka DNA-da ee la soo saaray waa mid weyn, nadiif ahaan aad u sarreeya, tayo ahaan deggan oo la isku halayn karo, cabbirka jajabka DNA-ga waa mid deggan qiyaastii 23kb.
Qalabka waxa kale oo loo isticmaali karaa in laga soo saaro unugyo kale oo jiirka ah oo ka baxsan dabada jiirka, oo ay ku jiraan dhegaha jiirka, muruqa jiirka, beerka jiirka iyo unugyo kale, si hal xidhmo loo isticmaalo ujeedooyin badan oo uu daboolo baahida isticmaalayaasha tijaabooyin kala duwan.
Tilmaamaha
50 Diyaarin, 100 Diyaarin, 250 Diyaarin
Qaybaha kitka
| Buffer TL1 |
| Bakhaar TL2* |
| Buffer PW* |
| Buffer WB |
| Buffer EB |
| Foregene Protease Plus |
| Tiirka-Kaliya DNA |
| Tilmaamaha |
Tilmaamaha & faa'iidooyinka
-Ma jirto faddarayn RNase: Tiirka DNA-kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo laga fogaanayo shaybaarka in uu wasakheeyo RNase dibadda ah.
Xawaaraha degdega ah: Foregene Protease Plus waxa uu leeyahay hawl ka sarraysa borotiijiyeyaasha la midka ah, waxayna si dhakhso ah u dheefshiidaa muunada nudaha;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 40 daqiiqo gudahood.
-Ku haboon: Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi loo qabo 4°C heerkulka hoose ee centrifugation ama roobab ethanol ee DNA.
-Badbaadada: Soo saarista reagents organic looma baahna.
-Tayada sare: DNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, aan lahayn RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
Codsiga qalabka
Waxay ku habboon tahay soo saarista iyo nadiifinta DNA-da genomic ee cusub ama barafaysan dabo waaweyn iyo jiirka (sidoo kale loo isticmaalo sifaynta jiirka ama murqaha jiirka iyo jiirka dhegta genomic DNA).
Socodka shaqada
Kaydinta iyo nolosha shelf
-Qalabkan waxaa lagu kaydin karaa 12 bilood xaaladaha qalalan ee heerkulka qolka (15-25 ° C);Haddii loo baahdo in lagu kaydiyo wakhti dheer, waxa lagu kaydin karaa 2-8 ° C.
Fiiro gaar ah: Haddii lagu kaydiyo heerkul hooseeya, xalku wuxuu u nugul yahay roobab.Kahor intaan la isticmaalin, hubso inaad geliso xalka qalabka heerkulka qolka muddo wakhti ah.Haddii loo baahdo, ku sii kululee biyaha qubeyska 37°C 10 daqiiqo si aad u milanto daadka, oo ku qas kahor isticmaalka.
Xalka Foregene Protease Plus wuxuu leeyahay qaacido gaar ah, waa firfircoon marka lagu kaydiyo heerkulka qolka muddo dheer (3 bilood);lagu kaydiyo 4°C, dhaqdhaqaaqa iyo xasiloonida ayaa fiicnaan doona, sidaa awgeed waxa lagu talinayaa in lagu kaydiyo 4°C, xasuuso inaadan ku haynin -20°C.
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