RT-PCR Easyᵀᴹ II (Laba Talaabo)
Sharaxaada Qalabka:
◮Falanqaynta tirada waqtiga-dhabta ah ee RNA si dhakhso ah oo sax ah ayaa loo samayn karaa.
◮Qalabku waxa uu isticmaalaa reagent transcription u gaar ah Foregene iyo Foregene HotStar Taq DNA Polymerase oo ay weheliso nidaam falcelin gaar ah si si wax ku ool ah loo wanaajiyo waxtarka xoojinta iyo gaar ahaaneed ee falcelinta.
◮ Habka falcelinta ee la wanaajiyey ayaa ka dhigaysa fal-celintu inay yeelato dareen-helid sare, degganaansho kulayleed oo xooggan, iyo dulqaad wanaagsan.
Tilmaamaha
RT-PCR FududTM II(Laba Talaabo) Waxay ka kooban tahay dhammaan reagen-yaasha laba-tallaabo ee RT-PCR, isku darka falcelinta RT iyo falcelinta PCR ee isla xirmada, taasoo bixisa isku-dhafka RT-ka iyo PCR-ga la hagaajiyay.Isku-dhafka RT-ga gaarka ah wuxuu si fudud oo hufan u soo saari karaa cDNA-yada koowaad ee tayada sare leh. 2× PCRFududTM Isku qasDheeraad ah waxay leedahay hufnaan sare iyo cod-weyneyn gaar ah.Isku dhafka dabiiciga ah ee labada ayaa ka dhigaya ogaanshaha hidda-socodka bartilmaameedka mid xasaasi ah.
Qaybaha kitka
| RT-PCRFududTM II (Laba Talaabo) | ||
| Qalabka ku jira | RT-02021 | RT-02022 |
| 100T(20 mlnidaamka) | 500T(20 mlnidaamka) | |
| 2× RT FududTM Isku qas | 1ml | 1.7ml×3 |
| 2× PCRFududTM Isku qasDheeraad ah | 1ml | 1.7ml×3 |
| Random Primer (50μM) | 200μl | 1ml |
| Oligo(dT)18 Hordhac (50μM) | 200μl | 1ml |
| RNase-free ddH2O | 1.7ml | 1.7ml |
| Buugga tilmaamaha | 1gabal | 1gabal |
Tilmaamaha & faa'iidooyinka
-Falanqaynta tirada waqtiga-dhabta ah ee RNA si dhakhso ah oo sax ah ayaa loo samayn karaa.
-Qalabku waxa uu isticmaalaa reagent transcription u gaar ah Foregene iyo Foregene HotStar Taq DNA Polymerase oo ay weheliso nidaam falcelin gaar ah si si wax ku ool ah loo wanaajiyo waxtarka xoojinta iyo gaar ahaaneed ee falcelinta.
-Habka falcelinta ee la wanaajiyey ayaa ka dhigaysa fal-celintu inay yeelato dareen-helid sare, degganaansho kulayleed oo xooggan, iyo dulqaad wanaagsan.
Socodka shaqada
Kaydinta iyo nolosha shelf
1.Xaaladaha gaadiidka
Geedi socodka oo dhan ee sanduuqa barafka heerkulka hooseeya, si loo hubiyo in qalabku ku jiro xaalad <4 °C.
2. Xaaladaha kaydinta
Qalabka waxaa lagu kaydiyaa heerkulka -20 ° C.Ku kaydi alaabta qaboojiyaha heerkulka joogtada ah -20 ° C isla markiiba ka dib markii la helo.Haddii shuruudaha kaydinta ay habboon yihiin,talaabtu ma hoos u dhigi doonto wax qabad kasta inta lagu jiro muddada ansaxinta 1-sano.
