EndoFree Maxi Plasmid Kit (Qalabka Spin)
Sharaxaada Qalabka:
Nidaamka soo saarista oo dhan wuxuu qaadanayaa 1 saac oo keliya, isagoo hubinaya in ku habboon oo degdeg ah hawlgalka.
Sharaxaada
Qalabka EndoFree Maxi Plasmid wuxuu qaataa farsamada xayeysiinta xuub silica gaar ah si si hufan oo gaar ah loogu xiro DNA-da plasmid.Marka la isku daro Endotoxin Removal Buffer P4 iyo EndoFree Maxi Filtration Columns, endotoxin, borotiinno iyo wasakh kale ayaa si wax ku ool ah looga saari karaa.Nidaamka soo saarista oo dhan wuxuu qaadanayaa 1 saac oo keliya, isagoo hubinaya in ku habboon oo degdeg ah hawlgalka.
Bakteeriyada lagu taliyay hawl kasta: plasmid-koobi sare leh, waxa lagu talinayaa in la isticmaalo 100 ml oo ah warbaahinta dhaqanka bakteeriyada, iyo wax-soo-saarka guud ahaan waa ku dhawaad 500-1500 μg.plasmid-koobi hooseeya, 200 ml warbaahinta dhaqanka bakteeriyada ayaa lagula talinayaa in ay dhaliso ilaa 200-600 μg plasmids.
Qaybaha kitka
| Alaabta Qaybaha | D909-E(10 diyaarin ah) |
| Buffer BL | 30 ml |
| Buffer P1 | 100 ml |
| Buffer P2 | 100 ml |
| Buffer P4 | 100 ml |
| Buffer DW2 | 44 ml |
| Buffer EB | 30 ml |
| RNase A (10 mg/ml) | 1 ml |
| DNAPure Maxi Spin Columns | 10 pcs |
| Saliingaha Filter ee FinePure Maxi | 10 pcs |
| 50 ml Tubooyinka Ururinta | 10 pcs |
Ogaanshaha Isku-duubnaanta DNA-da Plasmid iyo Nadiifinta
Nadiifinta iyo xoogga saaridda DNA-da plasmid-ka ee la soo saaray waxaa lagu ogaan karaa agarose gel electrophoresis iyo spectrophotometer ultraviolet.Qiimaha OD260 ee 1 wuxuu u dhigmaa ku dhawaad 50 μg/ml ee DNA labanlaaban.Qiimaha OD260/OD280 ee DNA plasmid-ka la safeeyey ayaa guud ahaan ku jira inta u dhaxaysa 1.7-1.9.DNA-da la safeeyey ee plasmid-ka ayaa si toos ah loogu dabaqi karaa tijaabooyinka shuruudaha nadaafadda sare, sida isu gudbinta unugyada ama tijaabooyinka vivo.
Codsiga qalabka
DNA-da plasmid-ka ee laga soo saaro xirmadan waxaa lagu dabaqi karaa hawlgallo kala duwan oo joogto ah, oo ay ku jiraan dheefshiidka enzymatic, PCR, sequencing, ligation, isbeddelka iyo sidoo kale gudbinta noocyada kala duwan ee unugyada.
Kaydinta iyo nolosha shelf
Qalabka EndoFree Maxi Plasmid waxaa lagu kaydin karaa heerkulka qolka (15-25°C) ee xaaladaha qalalan ilaa 12 bilood.Ku dar RNase A Buffer P1 ka hor isticmaalka koowaad, si fiican isku qas oo ku kaydi 2-8°C.Isku darka waxa loo kaydin karaa si adag in ka badan 6 bilood.
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