RT-qPCR Easyᵀᴹ (Hal Talaabo) -SYBR Cagaaran I
Sharaxaada Qalabka:
◮Xirmada hal-tallaabo waxay samaysaa qoraal celin iyo qPCR laba falcelin oo isla tuubo ah, kaliya waxay u baahan yihiin in lagu daro template RNA, asal PCR gaar ah iyo RNase-free ddH2O.
◮Xirmada ayaa si degdeg ah oo hufan u baari karta RNA fayraska ama raadraaca RNA.
◮Qalabku waxa uu isticmaalaa reagent transcription u gaar ah Foregene iyo Foregene HotStar Taq DNA Polymerase oo ay weheliso nidaam falcelin gaar ah si si wax ku ool ah loo wanaajiyo waxtarka xoojinta iyo gaar ahaaneed ee falcelinta.
◮Habka falcelinta ee la wanaajiyey ayaa ka dhigaysa fal-celintu inay yeelato dareen-helid sare, degganaansho kulayleed oo xooggan, iyo dulqaad wanaagsan.
◮RT-qPCR FududTM(Hal Tallaabo) -SYBR Cagaaran I xirmada waxay la socotaa ROX dheeha tixraaca gudaha, kaas oo loo isticmaali karo in lagu baabi'iyo asalka calaamadaha iyo khaladaadka u dhexeeya ceelasha, taas oo ku habboon macaamiisha inay u isticmaalaan noocyo kala duwan oo qalab PCR ah.
Sharaxaada
RT-qPCR FududTM (Hal Tallaabo)-SYBR Cagaaran I adeegsada nidaam gaar ah si uu si wax ku ool ah isugu daro RT iyo Real Time falcelinta PCR, kaas oo si dhakhso ah u dhamaystiri kara ogaanshaha tirada hiddo-wadaha.Alaabtu waxay leedahay dulqaad xooggan, gaar gaar ah iyo xasilooni.Alaabtu waxay ka kooban tahay shirkadda Foregene HotStar Taq DNA Polymerase ee gaarka ah, kaas oo leh faa'iidooyinka kor u qaadida degdega ah (2Kb/min), hufnaanta sare u qaadida, awood xoog leh oo gaar ah oo xoog leh, iyo isbarbardhig hooseeya oo hooseeya marka la barbar dhigo enzymes Taq caadiga ah.Waxa halkan loogu istcimaalaa RT-PCR-ka-dhabta ah si loo yareeyo kordhinta aan gaarka ahayn loona wanaajiyo saxnaanta PCR.
Tilmaamaha
100 T (Nidaamka 20μl)
500T (Nidaamka 20μl)
Qaybaha kitka
| RT-qPCR FududTM(Hal Tallaabo)-SYBR Cagaaran I | ||
| Tusmada Kit | RT-02111 | RT-02112 |
| 100T (Nidaamka 20μl) | 500T (Nidaamka 20μl) | |
| 2× RT-qPCR FududTM Isku-dhafka-SYBR | 1ml | 1.7ml×3 |
| 50× ROX Tixraaca Midabka | 200ml | 1ml |
| RNase-free ddH2O | 1.7ml | 1.7ml×3 |
| Buugga tilmaamaha | 1 gabal | 1 gabal |
Tilmaamaha & faa'iidooyinka
- Xidhmada hal-tallaabo ah waxay samaysaa qoraal-celin iyo qPCR laba falcelin oo isku mid ah tubbada, kaliya waxay u baahan yihiin in lagu daro template RNA, aasaasayaasha PCR gaar ah iyo RNase-free ddH2O.
- Xirmada ayaa si degdeg ah oo hufan u baari karta RNA fayraska ama raadraaca RNA.
- Xidhmadani waxay isticmaashaa reagent transcription u gaar ah Foregene iyo Foregene HotStar Taq DNA Polymerase oo ay weheliso nidaam falcelin gaar ah si ay si wax ku ool ah u wanaajiso waxtarka xoojinta iyo gaar ahaaneed ee falcelinta.
-Nidaamka falcelinta ee la wanaajiyey ayaa ka dhigaysa fal-celintu inay yeelato dareen ogaansho sare leh, degganaansho kulayleed oo xoog badan, iyo dulqaad wanaagsan.
- RT-qPCR FududTM(Hal Tallaabo) -SYBR Cagaaran I xirmada waxay la socotaa ROX dheeha tixraaca gudaha, kaas oo loo isticmaali karo in lagu baabi'iyo asalka calaamadaha iyo khaladaadka u dhexeeya ceelasha, taas oo ku habboon macaamiisha inay u isticmaalaan noocyo kala duwan oo qalab PCR ah.
Socodka shaqada
Xaaladaha kaydinta
1.Gaadiidkashuruudaha
Geedi socodka oo dhan ee sanduuqa barafka heerkulka hooseeya, si loo hubiyo in qalabku ku jiro xaalad <4 ℃ ah.
2.Kaydinta shuruudaha
Qalabka waxaa lagu kaydiyaa -20 ℃.Ku kaydi alaabta qaboojiyaha heerkulka joogtada ah at-20 ℃ isla markiiba ka dib markii la helo.Haddii shuruudaha kaydinta ay ku habboon yihiin, alaabtahoos u dhigi maayo waxqabad kasta inta lagu jiro mudada ansaxinta 1-sano ah.
