Abuurka Dhirta (weyn) Qalabka PCR Tooska ah II-UNG (aan lahayn Qalabka Muunalada)
Sharaxaada Qalabka:
Isticmaalka tooska ah ee abuurka lysate sida template for amplification PCR, ma soosaarid gaar ah ee DNA, degdeg ah iyo dareen sare.Nidaamka ka hortagga wasakheynta ee UNG ayaa lagu daray si looga hortago waxyaabo been abuur ah.
◮Looma baahna nadiifin waqti-qaadasho iyo qaali ah.
◮Dalabka muunadku wuu yar yahay, kaliya qaado hal abuur.
◮Looma baahna daaweyn gaar ah sida shiidi iyo burburin, qalliinkuna waa sahlan yahay.
◮Nidaamka PCR ee la hagaajiyay wuxuu awood u siinayaa PCR inuu yeesho gaar gaar ah iyo dulqaad adag oo loogu talagalay horjoogayaasha falcelinta PCR.
Sharaxaada
Alaabtani waxay isticmaashaa habka falcelinta lysis ee gaarka ah, kaas oo si dhakhso ah u sii deyn kara DNA-ga genomic ee shaybaarada abuurka dhirta leh polysaccharide iyo polyphenol hooseeya (sida: bariiska, sarreenka, tubaakada, galleyda, soybeans, iwm) si loogu isticmaalo falcelinta PCR, sidaas darteed waxay si gaar ah ugu habboon tahay baaritaanka hidda-socodka ballaaran.Si loo daboolo baahiyaha tijaabooyinka ogaanshaha kala duwan, xirmadani waxay adeegsan kartaa noocyo badan oo muunado ah (sida iniinaha oo dhan, goynta unugyada yaryar, ama muunadaha dhulka ee abuur badan)Waxaa ka mid ah, shaybaarada abuurka ee weli u baahan in la biqlo, waxaad dooran kartaa inaad gooyso unugyada unugyada yaryar (1-5mg) oo aan ahayn embriyaha abuurka si tijaabo ah;Tijaabooyin u baahan in lagu qaado muunado tiro badan, waxaad dooran kartaa inaad isku qasto muunado badan oo aad isku darsato.Ka dib marka la shiido qaybo leh dhexroor qiyaastii ah 0.5mm, tijaabada waa la samayn karaa (ugu yaraan 0.1% muunada bartilmaameedka ayaa la ogaan karaa).
Tilmaamaha
50T,200T,500T,2000T
Qaybaha kitka
| Abuurka Dhirta Qalabka PCR ee tooska ah II-UNG Abuurka Dhirta (weyn) Qalabka PCR Tooska ah-UNG | |||||
| Halabuurka Kit | TP-0314T | TP-03141 | TP-03142 | TP-03143 | |
| 50 T | 200T | 500T | 2000T | ||
| Qaybta I | Buffer SP1 | 30ml | 120ml | 100ml×3 | 400ml×3 |
| Buffer SP2 | 3ml | 10ml | 25ml | 100ml | |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
| Qaybta II | 2× Seed PCR EasyTMIsku qas(UNG) | 500ml | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
| Buugga | 1 | 1 | 1 | 1 | |
Tilmaamaha iyo faa'iidooyinka
- Looma baahna waqti-qaadasho iyo nadiifin DNA qaali ah ayaa loo baahan yahay.
- Baahida muunadku way yar tahay, kaliya qaado hal abuur.
- Looma baahna daawayn gaar ah sida shiidi iyo burburin, qalliinkuna waa sahlan yahay.
-Nidaamka PCR ee la wanaajiyey waxa uu awood u siinayaa PCR in uu yeesho gaar gaar ah iyo dulqaad adag oo loogu talagalay hor joogayaasha falcelinta PCR.
- Nidaamka PCR-ka-hortagga wasakheynta 2× Seed PCR EasyTMIsku-dhafka (UNG) wuxuu si wax ku ool ah u baabi'in karaa wasakhowga ay sababaan alaabada PCR wuxuuna hubin karaa gaar ahaan iyo saxnaanta xoojinta.
Socodka shaqada
Xaaladaha kaydinta
1.Gaadiidka
Geedi socodka oo dhan ee sanduuqa barafka heerkulka hooseeya, si loo hubiyo in xidhmada Qaybta II ay ku jirto xaalad <4 ℃ ah.
2.Kaydinta
Qaybta I ee xirmadan waa in lagu kaydiyaa heerkulka qolka ama 2-8°C.
Qaybta II waa in lagu kaydiyaa -20°C.
