Xirmada Go'doominta DNA ee Biyaha Xirmada Soosaarida iyo Sifaynta DNA ee Biyaha
Sharaxaada Qalabka:
Si deg deg ah uga nadiifi DNA-ga genomic-ga tayada sare leh ee ka yimaadda microorganisms ee muunado biyo oo kala duwan.
◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.
◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.
◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.
◮Badbaadada:Looma baahna soo saarista reagent organic.
◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.
Tilmaamaha
50 diyaarin
-Lysozyme: waxay enzymatically hydrolyze gidaarka unugga bakteeriyada togan.
-Buffer TE: waxaa loo isticmaalaa in lagu diyaariyo 100mg/ml xal Lysozyme iyo bixinta lysozyme enzymatic hydrolysis deegaanka.
-Buffer SG1 & Buffer SG2: Bixi muunad deegaan dheefshiidka borotiinka.
Protease-ka hore: Enzymatically hydrolyze shaybaarro ku jira jawi enzymatic-protease si loo sii daayo genomic DNA.
-Buffer SG3: Inactivate Foregene Protease oo bixiya bay'ad rarka DNA.
-Buffer SG4: Kaabista si loo bixiyo jawi rarka DNA.
-Buffer PW: Ka saar wasakhyada sida borotiinka iyo RNA ee DNA-da.
-Buffer WB: Ka saar ion milixda hadhaaga ah ee DNA-da.
-Buffer EB: ka saar DNA-da xuubka tiirka sifaynta.
-Tiirka-DNA-keliya: si gaar ah u dhaji DNA-da genomic ee lysate.
Qaybaha kitka
| Buffer SG1 |
| Buffer SG2 |
| Buffer SG3 |
| Buffer SG4 |
| Buffer PW |
| Buffer WB |
| Buffer EB |
| Buffer TE |
| Kahortagga Protease |
| Lysozyme |
| Tiirka-Kaliya DNA |
| Tilmaamaha |
Tilmaamaha & faa'iidooyinka
-Ma jirto faddarayn RNase: Tiirka DNA-kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan la helin RNase dheeraad ah inta lagu jiro tijaabada, iyada oo laga fogaanayo shaybaarka in uu wasakheeyo RNase dibadda ah.
Xawaaraha degdega ah: Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga biyaha lagu dhamayn karaa 40 daqiiqo gudahood.
-Ku haboon: Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi loo qabo 4°C heerkulka hoose ee centrifugation ama roobab ethanol ee DNA.
-Badbaadada: looma baahna soo saarista reagents organic.
-Tayada sare: Qaybaha DNA-da ee la soo saaray waa waaweyn yihiin, ma jiraan RNA, ma jiro RNase, iyo maadada ion aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
Codsiga qalabka
Waxay ku habboon tahay nadiifinta DNA-da genomic ee shaybaarrada soo socda: biyaha balliga aquaculture, biyaha balliga, biyaha tuubada, biyaha wabiga, biyaha balliga ee lotus iyo muunado kale.
Socodka shaqada
Kaydinta iyo nolosha shelf
-Qalabkan waxa lagu kaydin karaa 12 bilood marka ay jiraan xaalado qallalan oo heerkul ah (15-25°C), haddii loo baahdo in lagu kaydiyo wakhti dheer waxa lagu kaydin karaa 2-8°C.
Fiiro gaar ah: Haddii lagu kaydiyo heerkul hooseeya, xalku wuxuu u nugul yahay roobab.Kahor intaan la isticmaalin, hubso inaad geliso xalka qalabka heerkulka qolka muddo wakhti ah.Haddii loo baahdo, ku sii kululee 37°C biyaha qubeyska ilaa 10 daqiiqo si aad u milmaan roobka, oo ku qas ka hor isticmaalka.
-Xalka Foregene Protease wuxuu leeyahay qaacido gaar ah, kaas oo firfircoon marka lagu kaydiyo heerkulka qolka muddo dheer (3 bilood);marka lagu kaydiyo 4℃, hawlihiisa iyo xasiloonida ayaa fiicnaan doona, markaa waxa lagu talinayaa in lagu kaydiyo 4℃, xusuusnow inaadan ku kaydin -20℃.
-Budada qalalan Lysozyme waxa lagu kaydiyaa -20°C;Xalka Lysozyme ee la diyaariyey ayaa loo qaybiyaa qaybo yaryar waxaana lagu kaydiyaa -20 ° C.
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