Nidaamka Aqoonsiga DNA ee Foregene 20A ( Soo saarista DNA-da bilaashka ah)
Sharaxaada Qalabka:
Nidaamka aqoonsiga DNA-da ee Foregene 20A wuxuu isticmaalaa tignoolajiyada calaamadaynta fluorescent shan midab leh si ay isku mar u xoojiso 19 STR loci iyo 1 loci galmo oo hal tuubo ah.Marka la daboolayo 13 CODIS core loci, 20 core loci ayaa buuxinaya shuruudaha Wasaaradda Amniga Dadweynaha ee xirmada STR ee iswada.Waa gacanta midig ee dhismaha xogta DNA-da ee dembi-baarista iyo aqoonsiga qaraabada garsoorka.
Sharaxaada
Nidaamka aqoonsiga DNA-da ee Foregene 20A wuxuu isticmaalaa tignoolajiyada calaamadaynta fluorescent shan midab leh si ay isku mar u xoojiso 19 STR loci iyo 1 loci galmo oo hal tuubo ah.Marka la daboolayo 13 CODIS core loci, 20 core loci ayaa buuxinaya shuruudaha Wasaaradda Amniga Dadweynaha ee xirmada STR ee iswada.Waa gacanta midig ee dhismaha xogta DNA-da ee dembi-baarista iyo aqoonsiga qaraabada garsoorka.
Waxyaabaha ku jira Kit
| Qalabka Componet | Magaca Componet | Mugga (μl / tube) | Tirada (tube) |
| PCR falcelinta kahortaga | 2.5×PCR falcelinta Ⅲ | 1000 | 2 |
| 5×20A aasaasiga ah | 500 | 2 | |
| Xakamaynta DNA 9947A (1ng/μl) | 25 | 1 | |
| Biyo dheecan | 1700 | 2 | |
| Taq Polymerase | Taq polymerase Ⅲ | 40 | 2 |
| PCR Rear-reaction reagent | Salaanka Allelic20A | 40 | 1 |
| Miisaanka molecular standardORG 500 | 150 | 2 | |
| Ogow | |||
Falanqaynta Xogta
1. Fur barnaamijka aqoonsiga Genemapper.Markaad isticmaalayso xirmadan markii ugu horeysay, waxaad u baahan tahay inaad soo dejiso baallayaasha, bin set, habka falanqaynta u dhigma, iyo heerka cabbirka 0, 490, 500)
2. Soo rar xogta electrophoresis, dooro cabbirada falanqaynta ee u dhigma sida guddi, habka falanqaynta, iyo heerka cabbirka, oo u beddel nooca muunadda ee Salaanka "Allelic Ladder" ee "Type Tusaalaha" tiirka;bilow falanqaynta xogta.
Kaydinta Reagent
1. Ka dib marka la helo xirmada lagu qaboojiyey baraf qallalan ama bac qallalan, haddii aan si ku meel gaar ah loo isticmaalin, fadlan ku kaydi meel ka hooseysa -20°C muddo dheer.
2. Kadib marka xirmada la soo saaro oo la isticmaalo, xirmada waa in lagu kaydiyaa 4°C kahor falcelinta si looga fogaado baraf soo noqnoqda iyo dhalaalidda;Insaymamka Taq waa in lagu kaydiyaa -20°C.
3. falcelinta ka dib, xirmada (oo ay ku jiraan miisaanka molecular ee heerka gudaha iyo jaranjarada) waa in lagu kaydiyaa "qolka ogaanshaha electrophoresis" ee 4°C, iska ilaali qaboojinta soo noqnoqda iyo dhalaalida, kana fogow xidhiidhka xirmada falcelinta kahor si looga fogaado wasakhda.
