Nidaamka Aqoonsiga DNA ee Foregene 27Y
Sharaxaada Qalabka:
Foregene Nidaamka Aqoonsiga DNA 27Y wuxuu isticmaalaa tignoolajiyada calaamadaynta fluorescent ee lix midab leh si uu u kordhiyo 27 Y-STR loci hal mar.Waxaa ka mid ah: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS448, DYS533, DYFY387 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Qalabku wuxuu leeyahay awood ka hortag ah oo wanaagsan, wuxuuna si toos ah u kordhin karaa waraaqaha shaandhada, kaarka FTA, suufka suufka, kaarka candhuufta, suufka afka, iyo sidoo kale waxaa loo isticmaali karaa in laga soo saaro oo lagu nadiifiyo qaababka DNA-da ee alaabta kiiska.
Sharaxaada
Foregene Nidaamka Aqoonsiga DNA 27Y wuxuu isticmaalaa tignoolajiyada calaamadaynta fluorescent ee lix midab leh si uu u kordhiyo 27 Y-STR loci hal mar.Waxaa ka mid ah: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS448, DYS533, DYFY387 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Qalabku wuxuu leeyahay awood ka hortag ah oo wanaagsan, wuxuuna si toos ah u kordhin karaa waraaqaha shaandhada, kaarka FTA, suufka suufka, kaarka candhuufta, suufka afka, iyo sidoo kale waxaa loo isticmaali karaa in laga soo saaro oo lagu nadiifiyo qaababka DNA-da ee alaabta kiiska.
Waxyaabaha ku jira Kit
| Qalabka tijaabada reagent | Magaca qaybta | Mugga (μl/tuubo) | Tirada (tuubo) |
| Qalabka falcelinta kahortagga PCR | 2×PCR Reaction Master Mix | 1250 | 2 |
| 5 x 27Y Primer Mix | 500 | 2 | |
| Heerka Qoraalka DNA-da 9948 | 20 | 2 | |
| Biyo dheecan | 1700 | 2 | |
| Xirmada falcelinta PCR ka dib | 27Y allele mix Salaanka | 20 | 2 |
| Heerka ORG500 Miisaanka molecular ee Orange | 150 | 2 |
Falanqaynta Xogta
1. Fur barnaamijka aqoonsiga Gene Mapper.Markaad isticmaalayso qalabkan markii ugu horeysay, waxaad u baahan tahay inaad soo dejiso baallayaasha, bin set, habka falanqaynta u dhiganta, iyo heerka cabbirka 0, 490, 500).
2. Soo rar xogta electrophoresis, dooro cabbirada falanqaynta ee u dhigma sida guddi, habka falanqaynta, iyo heerka cabbirka, oo u beddel nooca muunadda ee Salaanka "Allelic Ladder" ee "Type Tusaalaha" tiirka;bilow falanqaynta xogta.
Kaydinta Reagent
1. Fadlan ku kaydi meel ka hooseysa -20°C (haddii aan si ku meel gaar ah loo isticmaalin), ka dib markaad hesho xirmooyinka lagu qaboojiyey baraf qallalan ama xirmooyinka barafka jel.
2. Fadlan ku kaydi xirmada 4°C ka hor falcelinta si aad uga fogaato baraf soo noqnoqda iyo dhalaalidda, ka dib marka qalabka loo soo saaro isticmaalka,;enzyme waa in lagu kaydiyaa heerkulka -20 ° C.
2. falcelinta ka dib, xirmada (oo ay ku jiraan miisaanka molecular miisaanka gudaha heerka iyo jaranjarada) waa in lagu kaydiyaa "qolka ogaanshaha electrophoresis" ee 4 ° C, iska ilaali qaboojinta iyo dhalaalidda soo noqnoqda, kana fogow xiriirka xirmada falcelinta ka hor si looga fogaado wasakhda.
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