Nidaamka Aqoonsiga DNA ee Foregene 25A-C (Kiiska)
Sharaxaada Qalabka:
Nidaamka Aqoonsiga DNA-da ee Foregene 25A-C waxay isticmaashaa tignoolajiyada calaamadaynta fluorescence lix midab leh si ay u xoojiso 23 autosomal STR loci, hal Amel loci, hal Yindel loci iyo laba goob xakamaynta tayada gudaha markiiba si loo kordhiyo habab DNA-da oo laga soo saaro oo laga nadiifiyo muunado kiis.
Sharaxaada
Nidaamka Aqoonsiga DNA-da ee Foregene 25A-C waxay isticmaashaa tignoolajiyada calaamadaynta fluorescence lix midab leh si ay u xoojiso 23 autosomal STR loci, hal Amel loci, hal Yindel loci iyo laba goob xakamaynta tayada gudaha markiiba si loo kordhiyo habab DNA-da oo laga soo saaro oo laga nadiifiyo muunado kiis.
Waxyaabaha ku jira Kit
Diyaarinta nidaamka xoojinta
Halabuurka Diyaarinta ee nidaamka soo saarista iyo kordhinta caadiga ah
| Qaybaha | Nidaamka 10 μl (μl) | |
| Master Mix | 2.5×PCR Premix C Ⅰ | 4.0 |
| 10×25A-CPrimer Isku-dhafka | 1.0 | |
| Qaabka DNA-da | 1-5 | |
| Biyo dheecan | Samee mugga falcelinta oo ah 10μl |
Falanqaynta Xogta
Sawirka Lifaaqa 1:Genotyping jaranjarada ee allele
Sawirka La Xidhiidhay 2:DNA-da ku-qorista heerka 9948khariidad
Kaydinta Reagent
1. Kadib markaad hesho xirmada, fadlan ku kaydi meel ka hooseysa -20℃ haddii aadan u isticmaalin wakhtiga la joogo.
2. Ka dib marka xirmada la soo saaro oo la isticmaalo, qaybta falcelinta ka hor ee PCR xalka premixed waxaa lagu kaydiyaa -20 ℃, iyo reagents ka hor inta hadhay ee falcelinta ka hor waxaa lagu kaydiyaa 4 ℃ si looga fogaado baraf soo noqnoqda - dhalaalid.Haddii qiyaasta kaliya ay yar tahay, waxaa lagu talinayaa in lagu kaydiyo -20 ° C ka dib baakooyinka.
3. Falcelinta ka dib, qaybaha waxaa lagu hayaa 4℃ si looga fogaado baraf-dhalaalid soo noqnoqda.Ha taaban reagents-ka-hortagga falcelinta si aad uga fogaato wasakhowga.
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