Nidaamka Aqoonsiga DNA ee Foregene 30A (aan-soosaar la'aan)
Sharaxaada Qalabka:
FOREGENE Nidaamka aqoonsiga DNA 30A wuxuu isticmaalaa tignoolajiyada calaamadaynta fluorescent-ka midabka lehweynayn 29autosomalSTR meesha, midAmel locus iyo hal Yindel locus hal mar, oo si toos ah u xoojin kara warqad shaandhaysa, FTAkaararka, suufka suufka, kaararka candhuufta,iyo msuuf ka baxsan.
Sharaxaada
FOREGENE Nidaamka aqoonsiga DNA 30A wuxuu isticmaalaa tignoolajiyada calaamadaynta fluorescent-ka midabka lehweynayn 29autosomalSTR meesha, midAmel locus iyo hal Yindel locus hal mar, oo si toos ah u xoojin kara warqad shaandhaysa, FTAkaararka, suufka suufka, kaararka candhuufta,iyo msuuf ka baxsan.
Waxyaabaha ku jira Kit
Diyaarinta Nidaamka Kordhinta
Jadwalka 1: Halabuurka diyaarinta ee falcelinta-weynaynta heerka (ka-soo-saar-la'aanta).
|
| Cdabadhilifyo | 25 nidaamka μl (μl) | 10 nidaamka μl (μl) |
| Master Mix | 4 × Master Mix VII | 6.25 | 2.5 |
| 5 x 30AIsku-dhafka koowaad | 5.0 | 2 | |
| Biyo dheecan | 13.75 | 5.5 | |
| Blood wasakh | Dhexroorka 1.2mm | Dhexroorka 1.2mm | |
| Tmugga falcelinta otal | 25 μl | 10 μl | |
Falanqaynta Xogta
Sawirka Lifaaqa 1:Khariidadda Tayaynta Salaanka Allelic
Sawirka La Xidhiidhay 2:DNA-da ku-qorista heerka 9948khariidad
Kaydinta Reagent
1. Fadlan ku kaydi meel ka hooseysa -20°C,,kadib marka aad hesho xirmooyinka lagu qaboojiyey baraf qallalan ama baraf-gel.
2. Fadlan ku kaydi qaybta falcelinta ka hor 4× premix VII at -20°C,kadib xirmada loo soo saaray isticmalkaHaddii qiyaasta kaliya ay yar tahay, waxaa lagu talinayaa in lagu kaydiyo -20 ° C ka dib marka la nadiifiyo.
3. Ku kaydi qaybaha falcelinta ka dib 4°C, iska ilaali qaboojinta iyo dhalaalidda soo noqnoqda, hana taaban reagents ka hor falcelinta si aad isaga ilaaliso wasakhda.
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