Nidaamka Aqoonsiga DNA ee Foregene Y Plus (aan-soo-saarid la'aan)
Sharaxaada Qalabka:
ForegeneHabka aqoonsiga DNA-da Y Plus waxay isticmaashaa tignoolajiyada calaamadaynta fluorescent lix midab leh si ay u kordhiso 20 Y koromosoom core loci, 15 loci doorbiday, 6 beddelka loci iyo 3 Y indel loci hal mar.
Sharaxaada
ForegeneHabka aqoonsiga DNA-da Y Plus waxay isticmaashaa tignoolajiyada calaamadaynta fluorescent lix midab leh si ay u kordhiso 20 Y koromosoom core loci, 15 loci doorbiday, 6 beddelka loci iyo 3 Y indel loci hal mar.
Waxyaabaha ku jira Kit
Diyaarinta nidaamka xoojinta
Thehalabuurka diyaarintaee standard(soosaar la'aan)nidaamka kor u qaadida
| Cdabadhilifyo | 25 μlnidaamka (μl) | 10 μl (μl) | |
| Master Mix | 4 × Master MixVII | 6.25 | 2.5 |
| 5×Y Plus Primer Mix | 5.0 | 2.0 | |
| Biyo dheecan | 13.75 | 5.5 | |
| Blood wasakh | Dhexroorka 1.2mm | Dhexroorka 1.2mm | |
| Tmugga falcelinta otal | 25 μl | 10 μl | |
Falanqaynta Xogta
Sawirka Lifaaqa 1:Khariidadda Tayaynta Salaanka Allelic
Sawirka La Xidhiidhay 2:DNA-da ku-qorista heerka 9948khariidad
Kaydinta Reagent
1. Fadlan ku kaydi meel ka hooseysa -20°C(haddii aan si ku meel gaar ah loo isticmaalin),kadib marka aad hesho xirmooyinka lagu qaboojiyey baraf qallalan ama baraf qallalan.
2. Fadlan ku kaydi qaybta falcelinta ka hor 4× premix VII at -20°C,kadib marka xirmada la soo saaro si loogu isticmaalo,iyo inta kale ee soo hadhay reagement ka hor waxaa lagu kaydiyaa 4°C si looga fogaado baraf soo noqnoqda iyo dhalaalidda.Haddii qiyaasta kaliya ay yar tahay, waxaa lagu talinayaa in lagu kaydiyo -20 ° C ka dib marka la nadiifiyo.
3. Ku kaydi qaybaha falcelinta ka dib 4°C, iska ilaali qaboojinta iyo dhalaalidda soo noqnoqda, hana taaban reagents ka hor falcelinta si aad isaga ilaaliso wasakhda.
