Lnc-RT Heroᵀᴹ I (oo leh gDNase)
Tilmaamaha
25×20μl Rxns, 50×20μl Rxns
Lnc-RT HeroTM I (oo leh gDNase) waa nidaam qoraal ah oo si gaar ah loogu sameeyay lncRNA si dhakhso ah looga saaro wasakheynta DNA-ga.5×gDNase Mix waxay si dhakhso ah uga saari kartaa genome-ga haray ee RNA 42°C ilaa 2 daqiiqo, iyada oo si wax ku ool ah uga fogaanaysa faragelinta genome ee natiijooyinka qPCR.
5 × L-RT HeroTM Mix waxa ku jira Foregene LncRNA Reverse Transcriptase oo ay si gaar ah u horumarisay Foregene, kaas oo ah nooc cusub oo transcriptase ah oo gaar ah oo loogu talagalay lncRNA iyo qaabab kale oo kakan RNA oo dheer, oo leh xidhiidh RNA ka xoog badan iyo hufnaanta duubista ee sare.Nadaamka la hagaajiyay ayaa ka dhigaya heerka gadaasha qoraalka si dhakhso ah wuxuuna si fudud u qori karaa qaab-dhismeedka RNA oo leh ka kooban GC sare iyo qaab dhismeed sare oo adag.Xadhiga ugu horreeya ee cDNA wuxuu ku dhammayn karaa 42°C 15 daqiiqo gudahood.
Qaybaha kitka
| 5× Isku-dhafka gDNase |
| 5× L-RT HeroTM Isku-dhafka |
| RNase-free ddH2O |
| Tilmaamaha |
Tilmaamaha & faa'iidooyinka
■Awood hufan oo meesha looga saaro gDNA, kaas oo ka saari kara gDNA qaab-dhismeedka 2 daqiiqo gudahood.
■ Waxay si hufan u rogi kartaa qoraalka lncRNA.Marka badeecada qoraalka dib loo dhigo qPCR, qiimaha Ct wuu ka hooseeyaa midka caadiga ah ee habka qoraalka qoraalka.
■ Nidaamka qoraalka dib u celinta ee waxtarka leh, kaliya waxay qaadataa 15 daqiiqo in la dhamaystiro isku dhafka cDNA-ga ugu horreeya.
■ Qaababyo isku dhafan: qaab-dhismeedka ka kooban GC-ga sare iyo qaabdhismeedka sare ee adag ayaa sidoo kale lagu beddeli karaa hufnaan sare.
■ Nidaamka qoraalka dib u celinta dareenka sare, jaantusyada heerka pg sidoo kale waxay heli karaan cDNA tayo sare leh.
■ Nidaamka qoraalka dib u celinta wuxuu leeyahay degganaansho kulayl sare leh, heerkulka falcelinta ugu fiicani waa 42℃, oo wali waxay leedahay wax qabad wanaagsan oo qoraal ah oo 50 ah℃.
Codsiga qalabka
lncRNA qaraabo falanqaynta tirada.
lncRNA falanqaynta qiyaasta saxda ah.
Waxay si dhakhso ah oo sax ah u baari kartaa raadadka RNA sida fayraska RNA.
Dib u qorista qaab-dhismeedka RNA oo leh nuxurka GC sare ama qaabdhismeedka sare ee adag.
Socodka shaqada
Kaydinta iyo nolosha shelf
Qalabka waa in lagu kaydiyaa -20°C. Ku kaydi alaabta qaboojiyaha heerkulka joogtada ah ee -20°C isla markiiba ka dib markii la helo.Haddii shuruudaha kaydinta ay ku habboon yihiin, alaabtu ma dhimi doonto wax-qabad kasta inta lagu jiro muddada ansaxinta 1-sano.
Tilmaamaha Falanqaynta Dhibaatada
The following is an analysis of the problems that may be encountered in the use of Lnc-RT HeroTM I (With gDNase) series kits, hoping to be helpful to your experiments. In addition, we have dedicated technical support to help you with other experimental or technical problems beyond the operating instructions and questions. If you have any needs, please contact us: 028-83361257 or E-mail: Tech@foregene.com.
Non-xoojin gaar ah
1. Naqshadaynta asaasiga ah ee aan macquul ahayn
Talo-soo-jeedin: Naqshad naqshadeeyayaasha sida waafaqsan mabaadi'da naqshadaynta asaasiga ah.
2. Hadhaaga genome.
Soo jeedin: Hubi in talaabada ugu horeysa ee gDNA ka saarista heerkulka falcelinta ay tahay 42°C, wakhtiga falcelinta waxa kale oo la kordhin karaa ilaa 5min.
Ma jiro calaamad kor u qaada RT-qPCR
1. RNA waa la dhimay.
Talo soo jeedin: Walaxda soo saarista RNA waa inay noqotaa mid cusub intii suurtogal ah, iyo RNA tayo sare leh oo nadiif ah waa in la isticmaalaa.
2. RNA waxa ku jira inhibitors.
Talo-soo-jeedin: Ka-hortagga dib-u-celinta qoraalka guud ahaan waxaa ka mid ah SDS, cusbada guanidine, EDTA, iwm.
3. Arrimaha naqshadaynta aasaasiga ah.
Talo-soo-jeedin: Marka loo eego mabda'a naqshadaynta asaasiga ah, dib-u-qaabaynta aasaasayaasha kormeerka.
Kooxda bartilmaameedku waxay ka soo muuqanaysaa kantaroolka bannaan
1. Wasakhaynta qalabka hawlgalka ama reagents.
Talo soo jeedin: Dhammaan reage-yaasha ama qalabka tijaabada waa in si otomaatig ah loo xidho.Ka digtoonow oo si tartiib ah marka aad wax u qabanayso si aad uga hortagto in shaybaarka DNA-ga lagu soo nuugo tuubada ama laga daadiyo tuubada centrifuge.
2. Wasakhdu waxay dhacday intii lagu jiray diyaarinta nidaamka falcelinta PCR.
Talo soo jeedin: Qaado taxaddarrada lagama maarmaanka ah marka aad gacanta ku hayso, sida: xidhashada galoofyada cinjirka ah, adigoo isticmaalaya talooyinka shaandhada.U isticmaal PCR-ka-dhal ah habka-ka-hortagga wasakhda.
3. Asal-dhigayaashu way hoos u dhaceen.
Talo soo jeedin: Isticmaal SDS-PAGE electrophoresis si aad u ogaatid in furayaasha ay hoos u dhaceen iyo in kale, oo ku beddel furayaasha cusub ee tijaabooyinka ogaanshaha fluorescence.
Buugga tilmaamaha:
Lnc-RT HeroTM I (oo wata gDNase)
