Xirmada Go'doominta DNA ee FFPE Qalabka Sifaynta DNA ee Ka Soo Bixinta Tusaalooyinka FFPE
Sharaxaada Qalabka:
Si dhakhso leh uga nadiifi DNA-ga genomic-ga tayada sare leh unugyo ku-xidhan paraffin-ka sida qaybaha paraffin iyo blocks paraffin.
◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.
◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.
◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.
◮Badbaadada:Looma baahna soo saarista reagent organic.
◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.
Sharaxaada
Xirmadan waxaa loo qoondeeyay in laga soo saaro oo laga sifeeyo DNA-ga genomic oo tayo sare leh oo laga soo saaray unugyo sugan oo formaldehyde ah ama unug ku dhex jira paraffin.
DNA-da genomic ee muunada paraffin-ku-xidhan ayaa hoos u dhici doonta sababtoo ah wakhtiga kaydinta ama deegaanka, taas oo adkeynaysa in la soo saaro DNA-da genomic.Qalabkani waxa uu qaataa qaacido gaar ah oo la wanaajiyey, kaas oo ku habboon in laga soo saaro DNA-ga genomic ee shaybaarada paraffin-ku-xidhan ee leh heerka guusha soo saarista sare.Habka oo dhan uma baahna in la isticmaalo phenol sun ah, chloroform ama roobka isopropanol.
Xirmadani waxay isticmaashaa tiir lafdhabarta ah oo si gaar ah u xidhi kara DNA-da, nooc cusub oo Foregene Protease Plus ah, iyo nidaam kayd gaar ah, kaas oo soo saari kara DNA-ga unugyada unugyada paraffin-ku-xidhan oo tayo sare leh 2-5 saacadood gudahood.
Tilmaamaha
50 Diyaarin, 100 Diyaarin, 250 Diyaarin
Qaybaha kitka
| Buffer FL1 |
| Bakhaar FL2* |
| Buffer PW* |
| Buffer WB |
| Buffer EB |
| Foregene Protease Plus |
| Tiirka-Kaliya DNA |
| Tilmaamaha |
Tilmaamaha & faa'iidooyinka
-Ma jirto faddarayn RNase: Tiirka DNA-kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo laga fogaanayo shaybaarka in uu wasakheeyo RNase dibadda ah.
Xawaaraha degdega ah: Foregene Protease Plus waxa uu leeyahay hawl ka sarraysa borotiijiyeyaasha la midka ah, waxayna si dhakhso ah u dheefshiidaa muunada nudaha;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 2-5 saacadood gudahood.
- Ku habboonaanta: centrifugation waxaa lagu sameeyaa heerkulka qolka, loomana baahna 4°C centrifugation-ku-hooseeya ama roobab ethanol ee DNA.
-Badbaadada: soo saarista reagents organic looma baahna.
-Tayada sare: Qaybaha DNA-da ee la soo saaray waa waaweyn yihiin, ma jiraan RNA, ma jiro RNase, iyo maadada ion aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
Codsiga qalabka
Waxay ku habboon tahay soo saarista iyo nadiifinta DNA-da genomic ee shaybaarada formaldehyde-go'an iyo shaybaarada unugyada paraffin-ku-xidhan.
Socodka shaqada
Kaydinta iyo nolosha shelf
-Qalabkan waxa lagu kaydin karaa 12 bilood xaaladaha qalalan ee heerkulka qolka (15-25°C);Haddii loo baahdo in lagu kaydiyo wakhti dheer, waxa lagu kaydin karaa 2-8 ° C.
Fiiro gaar ah: Haddii lagu kaydiyo heerkul hooseeya, xalku wuxuu u nugul yahay roobab.Kahor intaan la isticmaalin, hubso inaad geliso xalka qalabka heerkulka qolka muddo wakhti ah.Haddii loo baahdo, ku sii kululee biyaha qubeyska 37°C 10 daqiiqo si aad u milanto daadka, oo ku qas kahor isticmaalka.
Xalka Foregene Protease Plus wuxuu leeyahay qaacido gaar ah oo firfircoon marka lagu kaydiyo heerkulka qolka muddo dheer (3 bilood);aad bay u firfircoon tahay oo xasilloon tahay marka lagu kaydiyo 4°C, sidaa awgeed waxa lagu talinayaa in lagu kaydiyo 4°C, xasuuso inaadan ku haynin -20°C.
