Xirmada Go'doominta DNA ee Saxarada Xirmooyinka Ka Soo Saarista Saxarada ee Saxarada
Sharaxaada Qalabka:
Si dhakhso leh u nadiifi oo hel DNA-ga hidde-ahaaneed oo tayo sare leh oo laga qaado muunado saxaro kala duwan.
◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.
◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.
◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.
◮Badbaadada:Looma baahna soo saarista reagent organic.
◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.
Sharaxaada
Qalabkani wuxuu bixiyaa hab degdeg ah oo sahlan oo laga soo saaro DNA-da genomic ee ilaha kala duwan ee ciidda.Waxaa jira tiro badan oo ka hortag ah sida humic acid iyo ion bir ah oo ku jira muunadaha ciidda.Xitaa haddii walxahan ay ku jiraan xaddi yar oo DNA-da la safeeyey, waxay saameyn ku yeelan doonaan falcelinta hoose, sida PCR iyo xaddidaadda dheef-shiid kiimikaadka enzyme.Sidaa darteed, furaha lagu nadiifinayo DNA-da ciidda waa sida si wax ku ool ah looga saaro xannibayaasha ciidda.
Xirmadani waxay isticmaashaa tiirka-DNA-Kaliya oo si gaar ah ugu xidhi kara DNA-da, nooc cusub oo Foregene Protease ah iyo nidaam kayd gaar ah, kaas oo si wax ku ool ah uga saari kara hor-joogayaasha kala duwan ee ciidda iyada oo aan la soo saarin dareeraha organic ama ethanol iyo roobka isopropanol.Soo saarista DNA-da ee shaybaarada ciidda waxa lagu dhamayn karaa 90 daqiiqo.
Tilmaamaha
25 Diyaarin, 50 Diyaarin, 100 Diyaarin, 250 Diyaarin
Qaybaha kitka
| Buffer SL1 |
| Buffer SL2 |
| Buffer SL3 |
| Buffer PW |
| Buffer WB |
| Buffer EB |
| Buffer TE |
| Kahortagga Protease |
| Lysozyme |
| Tiirka-Kaliya DNA |
| Tilmaamaha |
Tilmaamaha & faa'iidooyinka
-Ma jirto faddarayn RNase: Tiirka DNA-kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, taas oo ka hortagaysa in shaybaarka uu wasakheeyo RNase-ga dibadda ah.
Xawaaraha degdega ah: Qalliinku waa sahlan yahay, iyo hawlgalka soo saarista DNA-da saxarada waxaa lagu dhamayn karaa 40 daqiiqo gudahood.
-Ku haboon: Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi loo qabo 4°C heerkulka hoose ee centrifugation ama roobab ethanol ee DNA.
-Badbaadada: Soo saarista reagents organic looma baahna.
-Tayada sare: DNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, aan lahayn RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
Codsiga qalabka
Waxay ku habboon tahay nadiifinta DNA-da genomic ee shaybaarrada soo socda: bani-aadmiga cusub ama barafaysan, lo'da, digaaga, jiirka iyo shaybaarrada kale ee saxarada.
Socodka shaqada
Kaydinta iyo nolosha shelf
-Qalabkan waxa lagu kaydin karaa 12 bilood xaaladaha qalalan ee heerkulka qolka (15-25°C);Haddii loo baahdo in lagu kaydiyo wakhti dheer, waxa lagu kaydin karaa 2-8 ° C.
Fiiro gaar ah: Haddii lagu kaydiyo heerkul hooseeya, xalku wuxuu u nugul yahay roobab.Kahor intaan la isticmaalin, hubso inaad geliso xalka qalabka heerkulka qolka muddo wakhti ah.Haddii loo baahdo, ku sii kululee biyaha qubeyska 37°C 10 daqiiqo si aad u milanto daadka, oo ku qas kahor isticmaalka.
-Foregene Protease Solution waxa uu leeyahay qaacido u gaar ah, kaas oo firfircoon marka lagu kaydiyo heerkulka qolka muddo dheer (3 bilood), firfircoonidiisa iyo xasilloonida ayaa fiicnaan doonta marka lagu kaydiyo 4 ° C, sidaas darteed waxaa lagula talinayaa in lagu kaydiyo 4 ° C, xusuusnow inaadan ku hayn -20 ° C.
Budada Lysozyme ee qallalan waxaa lagu kaydiyaa -20 ° C, xalalka Lysozyme ee la diyaariyey ayaa loo qaybiyaa qaybo yaryar waxaana lagu kaydiyaa -20 ° C.
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