Genomic DNA Micro Kit Micro Genomic DNA Mini Kit
Sharaxaada Qalabka:
Si dhakhso leh u nadiifi muunado yaryar si aad u hesho DNA-ga genomic oo tayo sare leh.
◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.
◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.
◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.
◮Badbaadada:Looma baahna soo saarista reagent organic.
◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.
Sharaxaada
Xirmadani waxay isticmaashaa tiirka DNA-kaliya oo si gaar ah u xidhi kara DNA-da, nooc cusub oo Foregene Protease ah iyo nidaam kayd gaar ah, kaas oo si hufan u soo saari kara muunado bayooloji oo kala duwan, sida cadadka dhiiga, unugyada, iyo xoolaha 20-80 daqiiqo gudahood.Unugyada, dhiig-baxa qallalan, suufka suufka ah ee kiliinikada, timaha timaha, iwm, ayaa la nadiifiyaa si loo helo DNA-ga hidde-side-sare oo tayo sare leh.
Tilmaamaha
50 diyaarin
Qaybaha kitka
| Buffer ST1 |
| Buffer ST2* |
| Buffer PW* |
| Buffer WB |
| Buffer EB |
| Kahortagga Protease |
| Tiirka-Kaliya DNA |
| Tilmaamaha |
Tilmaamaha & faa'iidooyinka
-Ma jirto faddarayn RNase: Tiirka DNA-kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, taas oo ka hortagaysa in shaybaarka uu wasakheeyo RNase-ga dibadda ah.
Xawaaraha degdega ah: Foregene Protease wuxuu leeyahay firfircooni ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.
-Ku haboon: Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi loo qabo 4°C heerkulka hoose ee centrifugation ama roobab ethanol ee DNA.
-Badbaadada: Soo saarista reagents organic looma baahna.
-Tayada sare: DNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, aan lahayn RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
-Nidaamka Micro-elution: Waxa ay kordhin kartaa fiirsashada DNA-da genomic, taas oo ku habboon in la ogaado ama la tijaabiyo hoose.
Codsiga qalabka
Waxay ku habboon tahay nadiifinta shaybaarrada soo socda ee DNA-da genomic: unugyada xayawaanka cusub ama la qaboojiyey, qadar cusub ama xinjirowga lidka ku ah oo lagu keydiyay dhiigga oo dhan, dhibco dhiig oo qallalan, unugyo dhaqan, unugyo microsection ah, timaha follicles, afka dhaqidda, iwm.
Socodka shaqada
Kaydinta iyo nolosha shelf
-Qalabkan waxa lagu kaydin karaa 12 bilood xaaladaha qalalan ee heerkulka qolka (15-25°C);Haddii loo baahdo in lagu kaydiyo wakhti dheer, waxa lagu kaydin karaa 2-8 ° C.
Fiiro gaar ah: Haddii lagu kaydiyo heerkul hooseeya, xalku wuxuu u nugul yahay roobab.Kahor intaan la isticmaalin, hubso inaad geliso xalka qalabka heerkulka qolka muddo wakhti ah.Haddii loo baahdo, ku sii kululee biyaha qubeyska 37°C 10 daqiiqo si aad u milanto daadka, oo ku qas kahor isticmaalka.
-Xalka Foregene Protease wuxuu leeyahay qaacido gaar ah, kaas oo firfircoon marka lagu kaydiyo heerkulka qolka muddo dheer (3 bilood);firfircoonideeda iyo xasilloonideeda waxay fiicnaan doontaa marka lagu kaydiyo 4°C, markaa waxaa lagu talinayaa in lagu kaydiyo4°C, xasuuso inaadan ku hayn heerkul -20°C.
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