RT-qPCR Easyᵀᴹ (Hal Talaabo) -Taqman
Sharaxaada Qalabka:
- Hal-Xirmada tillaabadu waxay awood u siinaysaa qoraal-celinta iyo PCR in lagu sameeyo isla tuubo, kaliya waxay u baahan yihiin in lagu daro template RNA, PCR-ga gaarka ah iyo ddH-RNase-free2O.
- Falanqaynta tirada waqtiga-dhabta ah ee fayraska RNA ama raadraaca RNA si dhakhso leh oo sax ah ayaa loo samayn karaa.
- Xidhmadani waxay isticmaashaa reagent transcription u gaar ah Foregene iyo Foregene HotStar Taq DNA Polymerase oo ay weheliso nidaam falcelin gaar ah si ay si wax ku ool ah u wanaajiso waxtarka xoojinta iyo gaar ahaaneed ee falcelinta.
-Nidaamka falcelinta ee la wanaajiyey ayaa ka dhigaysa fal-celintu inay yeelato dareen ogaansho sare leh, degganaansho kulayleed oo xoog badan, iyo dulqaad wanaagsan.
- RT-qPCR FududTM(Hal Talaabo) - Xirmada Taqman waxay la socotaa roodhida tixraaca gudaha ee ROX, kaas oo loo isticmaali karo in lagu tirtiro asalka calaamadaha iyo khaladaadka calaamadaha u dhexeeya ceelasha, taas oo ku habboon isticmaalayaasha dhamaadka inay u isticmaalaan noocyo kala duwan oo qalab PCR ah.
Sharaxaada
Foregene Hal-Tallaabo RT-PCR Alaabooyin taxane ah oo sahlan ayaa garwaaqsada falcelinta isku dhafan ee RNA ilaa DNA-da laba-geesoodka ah, taas oo ah, qoraal-celinta iyo kordhinta PCR-ga ayaa lagu dhammeeyaa isla tuubada centrifuge falcelinta iyo isla nidaamka falcelinta, sidaas awgeed tillaabooyinka tijaabada waa la fududeeyay, qorshaha tijaabada waa la hagaajiyay, iyo waxtarka tijaabada ayaa la hagaajiyay.
RT-qPCR FududTM (MidTallaabo)-Taqmaaniyadoo la adeegsanayo Foregene HotStar Taq DNA Polymerase, nidaamka la hagaajiyay ee Taqman qPCR wuxuu si degdeg ah oo gaar ah u fulin karaa waqtiga dhabta ah ee RT-qPCR ogaanshaha tirada jaantusyada RNA.
Tilmaamaha
100 Rxns (Nidaamka 20μl)
500 Rxns (Nidaamka 20μl)
Qaybaha kitka
| RT-qPCR EasyTM (Hal Talaabo) -Taqman | ||
| Qaybaha kitka | RT-02131 | RT-02132 |
| 100Rxns (Nidaamka 20μl) | 500 Rxns (Nidaamka 20μl) | |
| 2× RT-qPCR FududTMMix-Taqman | 1ml | 1.7ml×3 |
| 20× ROX Tixraaca Midabka | 100ml | 0.5ml |
| RNase-free ddH2O | 1.7ml | 1.7ml×3 |
| Tilmaamaha Isticmaalka | 1 | 1 |
Tilmaamaha & faa'iidooyinka
- Xirmada hal-tallaabo ah waxay awood u siinaysaa qoraal-celinta iyo PCR in lagu sameeyo isla tuubo, kaliya waxay u baahan tahay in lagu daro template RNA, aasaasiyaal PCR gaar ah iyo RNase-free ddH2O.
- Falanqaynta tirada waqtiga-dhabta ah ee fayraska RNA ama raadraaca RNA si dhakhso leh oo sax ah ayaa loo samayn karaa.
- Xidhmadani waxay isticmaashaa reagent transcription u gaar ah Foregene iyo Foregene HotStar Taq DNA Polymerase oo ay weheliso nidaam falcelin gaar ah si ay si wax ku ool ah u wanaajiso waxtarka xoojinta iyo gaar ahaaneed ee falcelinta.
-Nidaamka falcelinta ee la wanaajiyey ayaa ka dhigaysa fal-celintu inay yeelato dareen ogaansho sare leh, degganaansho kulayleed oo xoog badan, iyo dulqaad wanaagsan.
- RT-qPCR FududTM(Hal Talaabo) - Xirmada Taqman waxay la socotaa roodhida tixraaca gudaha ee ROX, kaas oo loo isticmaali karo in lagu tirtiro asalka calaamadaha iyo khaladaadka calaamadaha u dhexeeya ceelasha, taas oo ku habboon isticmaalayaasha dhamaadka inay u isticmaalaan noocyo kala duwan oo qalab PCR ah.
Xuduudaha Kit
Codsiga qalabka
- Qalabkan waxa loo isticmaalaa in si degdeg ah oo sax ah loo ogaado RNA-yada raadraaca sida fayraska RNA.
- Waxay si gaar ah ugu habboon tahay in si degdeg ah loo ogaado qaababka RNA oo leh maaddo GC sare ah ama qaab dhismeed sare oo adag.
Socodka shaqada
Xaaladaha kaydinta
1.Gaadiidkashuruudaha
Geedi socodka oo dhan ee sanduuqa barafka heerkulka hooseeya, si loo hubiyo in qalabku ku jiro xaalad <4 ℃ ah.
2.Kaydinta shuruudaha
Qalabka waxaa lagu kaydiyaa -20 ℃.Ku kaydi alaabta qaboojiyaha heerkulka joogtada ah at-20 ℃ isla markiiba ka dib markii la helo.Haddii shuruudaha kaydinta ay ku habboon yihiin, alaabtahoos u dhigi maayo waxqabad kasta inta lagu jiro mudada ansaxinta 1-sano ah.
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