Serum( Plasma) Qalabka Go'doominta miRNA
Sharaxaada Qalabka:
◆Xidhmada dhamaystiran ee RNase-Free, looma baahna in laga walwalo xaalufka RNA.
◆Tiirka-Nadiifinta DNA-da ayaa si gaar ah ugu xidhan DNA-da, si uu xidhmoku uga saaro wasakhaynta DNA-da iyada oo aan lagu darin DNA-da dheeraad ah.
◆Wax-soosaarka RNA sare: Tiirka-RNA-kaliya iyo qaacido gaar ah ayaa si hufan u nadiifin kara RNA.
◆Xawaaraha dheereeya: Qalliinku waa sahlan yahay waxaana lagu dhamayn karaa 30 daqiiqo gudahood.
◆Badbaadada: Looma baahna soo saarista reageent organic ah.
◆Tayo sare leh: Qaybaha RNA ee la soo saaray waxay leeyihiin nadiif sare, oo bilaash ah borotiin iyo wasakh kale, waxayna la kulmi karaan codsiyo tijaabo ah oo kala duwan.
Sharaxaada
Xirmadani waxay isticmaashaa tiirka lafdhabarta iyo caanaha ay samaysay shirkadeena, kaas oo si hufan u soo saari kara jajabyo yar yar oo miRNA ah oo ka yar 100nt serum iyo balasmaha.Qalabku wuxuu bixiyaa DNA-Cleaning Column, kaas oo si fudud u kala saari kara lysate-ka sare iyo unugyada iyo ka saarida DNA-da muunada, taas oo fudud oo waqti-badbaadinaysa;Tiirka RNA-keliya ayaa si hufan u xidhi kara RNA, iyo qaacido gaar ah, waxa la isticmaali karaa isku mar.Samee tiro badan oo muunado ah.
Nidaamka oo dhan waa RNase-free, si aan miRNA la safeeyey aan hoos loo dhigin;Buffer miBRW1 iyo Buffer miBRW2 nidaamka dhaqidda kaydka ayaa ka dhigaya RNA la helay iyada oo aan lahayn borotiin, DNA, ion, iyo wasakhaynta isku dhafka organic.
Tilmaamaha
5T, 50T, 100T, 200T
Qaybaha Kit
| Qalabka Go'doominta Serum/Plasma MiRNA | ||||
| Qalabka ku jira | RE-0111T | RE-01111 | RE-01112 | RE-01114 |
| 5 T | 50 T | 100T | 200T | |
| Buffer miBRL1* | 2ml | 20ml | 40ml | 80ml |
| Buffer miBRL2* | 1.2ml | 12ml | 24ml | 48ml |
| Buffer miBRW1 | 2ml | 20ml | 40ml | 80ml |
| Buffer miBRW2 | 2.4ml | 24ml | 48ml | 96ml |
| RNase-free ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| Tiirka RNA-kaliya | 5 gabal | 50gabal | 100gabal | 200gabal |
| Tiirka Nadiifinta DNA | 5 gabal | 50gabal | 100gabal | 200gabal |
| IFU | 1 gabal | 1 gabal | 1 gabal | 1 gabal |
Tilmaamaha & faa'iidooyinka
◆Xidhmada dhamaystiran ee RNase-Free, looma baahna in laga walwalo xaalufka RNA.
◆Tiirka-Nadiifinta DNA-da ayaa si gaar ah ugu xidhan DNA-da, si uu xidhmoku uga saaro wasakhaynta DNA-da iyada oo aan lagu darin DNA-da dheeraad ah.
◆Wax-soosaarka RNA sare: Tiirka-RNA-kaliya iyo qaacido gaar ah ayaa si hufan u nadiifin kara RNA.
◆Xawaaraha dheereeya: Qalliinku waa sahlan yahay waxaana lagu dhamayn karaa 30 daqiiqo gudahood.
◆Badbaadada: Looma baahna soo saarista reageent organic ah.
◆Tayo sare leh: Qaybaha RNA ee la soo saaray waxay leeyihiin nadiif sare, oo bilaash ah borotiin iyo wasakh kale, waxayna la kulmi karaan codsiyo tijaabo ah oo kala duwan.
Xuduudaha Kit
Codsiga Qalabka
Waxay ku habboon tahay soo saarista iyo nadiifinta miRNA ee xayawaanka iyo serum aadanaha iyo balasmaha.
Codsiga miRNA
MiRNA-ga lagu sifeeyey Serum/Plasma MiRNA Go'doominta MiRNA waxaa loo isticmaali karaa tijaabooyin kala duwan oo unugyo ah, sida: RT-PCR, Real Time RT-PCR, Northern Blot, falanqaynta chip, iwm.
Socodka shaqada
Xaaladaha kaydinta
Qalabkan waxaa lagu kaydin karaa 12 bilood xaaladaha qalalan ee heerkulka qolka(15-25°C).If waxay u baahan tahay in la kaydiyo wakhti dheerfadlanlagu kaydiyaa 2-8°C.
