Qalabka Go'doominta DNA-ga Dhirta Xirmooyinka Nadaafadda DNA-da ee Genomic Plant Protocol
Tilmaamaha
50 Diyaarin, 100 Diyaarin, 250 Diyaarin
Qalabkani waxa uu isticmaalaa tiir DNA-kaliya oo si gaar ah u xidhi kara DNA-da, Protease Foregene iyo nidaam kayd gaar ah, kaas oo si weyn u fududeeya nadiifinta DNA-da genomiga ee dhirta.DNA-ga genomic-ga tayada sare leh waxaa lagu heli karaa 30 daqiiqo gudahood, taas oo ka fogaanaysa hoos u dhaca DNA-da genomic.
Xuubka DNA-ga silica jel ee loo isticmaalo tiirarka lafdhabarta ayaa ah walaxda cusub ee Foregene, kaas oo si wax ku ool ah oo gaar ah ugu xidhi kara DNA-da, oo kor u qaadi karta ka saarida RNA, borotiinnada wasakhaysan, ions, polysaccharides, polyphenols iyo xeryahooda kale ee organic.
Qaybaha alaabta
| Buffer PL1, Buffer PL2 |
| Buffer PW, Buffer WB, Buffer EB |
| Kahortagga Protease |
| Tiirka-Kaliya DNA |
| Tilmaamaha |
Tilmaamaha & faa'iidooyinka
■ Wax faddarayn RNase ma leh: Tiirka DNA-kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan la helin RNase dheeraad ah inta lagu jiro tijaabada, taas oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.
■ Xawaaraha dheereeya: Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah wuxuuna si dhakhso ah u dheefshiidaa shaybaarka nudaha.
■ Fudud: hawlgalka soo saarista DNA-ga genomic waxa lagu dhamayn karaa 30 daqiiqo gudahood.
■ Ku habboon: Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, looma baahna 4℃ sentrifugation heerkulka hooseeya ama roobab ethanol ah oo loo baahan yahay.
■ Badbaadada: looma baahna reageent organic.
■ Tayada sare: DNA-da la safeeyey ee genomic waxay leedahay jajabyo waaweyn, malaha RNA, malaha RNase, iyo maadada ion aad u hooseysa, waxay buuxin kartaa shuruudaha tijaabooyinka kala duwan.
Codsiga qalabka
Ku habboon soo saarista iyo nadiifinta DNA-da genomic ee unugyada dhirta cusub ama barafaysan.
Socodka shaqada
Jaantuska
Kaydinta iyo nolosha shelf
Qalabka waxaa lagu kaydin karaa 12 bilood heerkulka qolka (15-25 ℃) iyo 2-8℃ waqti dheer.
Xalka Foregene Protease Plus wuxuu leeyahay qaacido gaar ah, kaas oo firfircoon marka lagu kaydiyo heerkulka qolka muddo dheer (3 bilood);firfircoonideeda iyo xasilloonideeda ayaa fiicnaan doonta marka lagu kaydiyo4℃, marka waxaa lagu talinayaa in lagu kaydiyo at 4℃, ha ku kaydin at -20 ℃.
Tilmaamaha Falanqaynta Dhibaatada
The following is an analysis of the problems that may be encountered in the extraction of plant genomic DNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mali: Tech@foregene.com.
Waxsoosaar hooseeya ama DNA-da oo aan lahayn
Badanaa waxaa jira arrimo badan oo saameeya soo-saarka DNA-da, oo ay ku jiraan isha muunadda, da'da muunada, xaaladaha kaydinta muunadda, iyo qalliinka.
DNA-da genomic lama heli karo inta lagu jiro soo saarista
1. Shaybaarada unugyada ayaa si khaldan loo kaydiyaa ama loo kaydiyaa wakhti aad u dheer, taasoo keentay hoos u dhaca DNA-da genomic.
Talo-soo-jeedin: Ku kaydi shaybaarada unugga ee dareeraha nitrogen ama -20°C;isku day inaad u isticmaasho muunado dhowaan la ururiyey si loo soo saaro DNA-da genomic.
2. Qadar muunad aad u yar ayaa laga yaabaa in ay keento in DNA-da genomiga ah ee u dhigma aan la soo saarin.
Talo-soo-jeedin: Shaybaarrada unugyada ee kaydsan muddo dheer ama leh burbur DNA-ga genomic oo daran, qaddarka shaybaarrada unugyada si habboon ayaa loo kordhin karaa si loo soo saaro DNA-ga genomic oo badan.Qadarka muunada waxaa lagu go'aamin karaa iyadoo loo eegayo baahida DNA-da, laakiin muunadda cusub waa inaysan dhaafin 100mg, muunadda qallalanna waa inaysan ka badnaan 30mg.
3. Muunada laguma darin nitrogen dareere ah ama laguma meeleeyo waqti aad u dheer ka dib nitrogen dareeraha ah.
Talo soo jeedin: Inta lagu jiro soo saarista DNA-da, muunadku waxa uu u baahan yahay in si buuxda loo geliyo nitrogen dareere ah si uu u jebiyo gidaarka unugga;Shiidi ka dib, fadlan ku wareeji budada muunada PL1 oo lagu sii kululeeyay 65°C sida ugu dhakhsaha badan (marka budada dhulka la dhalaaliyo, DNA-da genomic waxay bilaabi doontaa inay si degdeg ah u hoos u dhacdo).
4. Kaydinta khaldan ee Foregene Protease waxay keenaysaa hoos u dhac ama dhaqdhaqaaq la'aan.
Talo soo jeedin: Xaqiiji xaaladaha kaydinta Foregene Protease ama ku beddel Foregene Protease cusub oo loogu talagalay hydrolysis enzymatic.
5. Xirmada si aan habboonayn ayaa loo kaydiyaa ama loo kaydiyaa muddo aad u dheer, taasoo keenaysa in qaybo ka mid ah qalabkani ay fashilmaan.
Talo-soo-jeedin: Iibso xirmo soo saarista DNA-ga geedka cusub ee hawlaha la xiriira.
6. Isticmaalka aan habboonayn ee qalabka.
Soo-jeedin: Iibso Qalabka Go'doominta DNA-ga Dhirta oo u heellan shaybaarrada soo saarista iyo nadiifinta DNA-da hidde-sideyaasha.
7. Buffer WB adigoon ku darin anafaqo leh ethanol.
Talo soo jeedin: Hubi inaad ku darto mugga saxda ah ee ethanol buuxa ee Buffer WB.
8. Eluent si sax ah looguma daadin xuubka silica.
Soo jeedin: Ku dar eluent horay loo sii kululeeyay 65℃hoos u dhig ilaa dhexe ee xuubka silica jel, oo ku dhaaf heerkulka qolka 5 daqiiqo si loo kordhiyo waxtarka sare.
Soo saarista si loo helo DNA genomic-soo-saarka hooseeya
1. Tijaabada si aan habboonayn ayaa loo kaydiyaa ama loo kaydiyaa wakhti aad u dheer, taasoo keentay hoos u dhaca DNA-da genomic.
Talo soo jeedin: Ku kaydi shaybaarada unugyada at -20℃;isku day in aad isticmaasho muunado unug dhawaan la ururiyey si loo soo saaro DNA-da genomic.
2. Haddii cadadka shaybaarka unuggu aad u yar yahay, DNA-ga la soo saaray wuu yaraan doonaa.
Talo soo jeedin: Qaar ka mid ah muunadaha dhirta ayaa qani ku ah biyaha, sida dhirta biyaha sida algae, iwm., qiyaasta si habboon ayaa loo kordhin karaa ama biyaha ayaa fuuq-baxa ka hor qaliinka.
3. Tijaabooyinku si fiican looguma dhadhamin nitrogen dareere ah ama waxa lagu daayay heerkulka qolka muddo dheer ka dib marka la shiido.
Soo jeedin: Shiididda nitrogen dareeraha ah waa in ay ahaataa mid ku filan, iyo gidaarka muunada waa in la jebiyaa intii suurtagal ah;Isla markiiba ka dib shiididda, budada saamiga waa in loo wareejiyaa 65℃Preheated Buffer PL1 ee talaabada xigta.
4. Aan la isticmaalin qalabka saxda ah.
Talo-soo-jeedin: Isticmaal qalab go'doominta DNA-ga dhirta si aad u soo saarto oo aad u nadiifiso DNA-da hidde-sideyaasha.
5. Kaydinta khaldan ee Foregene Protease waxay keenaysaa hoos u dhac ama dhaqdhaqaaq la'aan.
Talo soo jeedin: Xaqiiji xaaladaha kaydinta Foregene Protease ama ku beddel Foregene Protease cusub oo loogu talagalay hydrolysis enzymatic.
6. Dhibaato ilbax ah
Talo soo jeedin: Fadlan u isticmaal Buffer EB si aad u qeexdo;haddii aad isticmaalayso ddH2O ama kuwa kale, hubi in pH-ga eluent-ku uu u dhexeeyo 7.0-8.5.
7. Ilmaha oo aan si sax ah loo dareerin
Talo soo jeedin: Fadlan ku dar dhibicda elutionka badhtamaha xuubka silica oo ku dhaaf heerkulka qolka 5 daqiiqo si loo kordhiyo waxtarka sare.
8. Mugga eluent aad buu u yar yahay
Soo jeedin: Fadlan u isticmaal eluent si aad u hesho DNA genomic sida waafaqsan tilmaamaha, ugu yaraan aan ka yarayn 100μl.
DNA-ga genomic ee la soo saaray oo leh daahir hoose
Nadiifinta hoose ee DNA-da genomic waxay u horseedi doontaa guuldarro ama saameyn liidata ee tijaabooyinka hoose, sida: enzyme lama gooyn karo, iyo jajabka hidda-wadaha bartilmaameedka ah ma heli karo PCR.
1. Wasakhaynta borotiinka kala duwan, wasakhaynta RNA.
Falanqaynta: Buffer PW looma isticmaalin in lagu dhaqo tiirka;Buffer PW looma isticmaalin in lagu dhaqo tiirka xawaaraha dhexe ee saxda ah.
Talo soo jeedin: isku day inaad hubiso inaysan jirin wax roobaad ah oo ka da'aya korka sare marka sareynta la dhex maro tiirka;Hubi inaad ku dhaqdo tiirka nadiifinta Buffer PW si waafaqsan tilmaamaha, tallaabadan lama dhaafi karo.
2. wasakhowga wasakhda wasakhaysan.
Falanqaynta: Tiirka dhaqidda ee Buffer WB waa laga saaray ama hal mar kaliya ayaa la dhaqay, taasoo keentay faddarayn ionic ah.
Talo soo jeedin: Hubi inaad laba jeer ku maydho Buffer WB sida ku cad tilmaamaha si aad meesha uga saarto ion haraaga intii suurtogal ah.
3. Wasakhda RNase.
Falanqaynta: Exogenous RNase waxaa lagu daraa kaydka;Hawlgalka dhaqidda khaldan ee Buffer PW wuxuu dhalin doonaa haraaga RNase wuxuuna saameeyaa hawlaha tijaabada ah ee RNA-da hoose, sida qorista vitro.
Soo jeedin: Xirmooyinka soo saarista aashitada nukliyeerka ee taxanaha ah ayaa ka saari kara RNA iyada oo aan la helin RNase dheeraad ah, iyo dhammaan reagen-yaasha ku jira Qalabka Go'doominta DNA uma baahna RNase;Hubi inaad ku dhaqdo tiirka nadiifinta Buffer PW si waafaqsan tilmaamaha, tallaabadan lama dhaafi karo.
4. Haraaga ethanol.
Falanqaynta: Ka dib markii lagu dhaqay tiirka sifaynta ee Buffer WB, ma jirto xarun dhexe oo tuubo oo madhan ah.
Talo-soo-jeedin: Raac tilmaan-bixinta ku-meel-gaadhka ah ee tuubbada madhan.
Buugga tilmaamaha:
Buugga Tilmaamaha Qalabka Go'doominta Dhirta ee DNA
