Qaybaha iyo Warshada Taxane ee Go'doominta DNA |Soosaarayaasha Taxanaha Go'doominta DNA ee Shiinaha

Qalabka Nadiifinta PCR Nidaamka Nadiifinta PCR ee DNA-da saafiga ah

Si dhakhso leh u nadiifi oo u hel jajabyada DNA-da tayada sare leh ee nidaamka PCR;Haddii aad rabto inaad kala tagto oo aad hesho jajab DNA oo gaar ah, fadlan dooro xirmada soo kabashada jel.

◮ Soo kabashada DNA-da oo ballaadhan: Qaybaha DNA-da oo gaaban sida 60bp iyo inta 10kb ah waa la soo ceshan karaa.

◮ Waxtarka soo kabashada sareWaxtarka soo kabashada guud ahaan waa in ka badan 80%, kan ugu sarreeyana wuxuu gaari karaa in ka badan 95%.

Xawaaraha dheereeya:Fudud in la shaqeeyo, soo kabashada jajabka DNA-da waxaa lagu dhameyn karaa 15 daqiiqo gudahood iyo dimer-ka si toos ah ayaa loo saari karaa iyada oo aan dib u soo kabashada jel.

◮ SafeefSoo saarista reagent organic looma baahna.

◮ Tayo sare: Qaybaha DNA-da ee la helay waa nadiif sare, kuwaas oo la kulmi kara tijaabooyin kala duwan oo xiga.

weydiin tafatiran
Xirmooyinka Go'doominta DNA-da bakteeriyada Bakteeriyada Genomic DNA Xirmooyinka Sifaynta

Si dhakhso leh uga nadiifi DNA-ga tayada sare leh ee bakteeriyada ee marxaladda koritaanka logarithmic.

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

weydiin tafatiran
Mouse Tail DNA Mini Kit Xirmooyinka Soo saarista DNA ee Genomic ee Dabada Mouse

Qalabka ugu dhaqsaha badan adduunka ee lagu soo saaro DNA-da dabada jiirka, kaas oo 1 saac gudaheed ka soo saari kara DNA-ga genomic-ga ah ee tayada sare leh.

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

weydiin tafatiran
Xirmada Soosaarida Gel-ga ee DNA-ga

Si dhakhso leh oo hufan uga soo kabso jajabyada DNA-da 20bp-10kb ee jelka agarose.

◮Soo kabashada DNA-da ballaaran:Qaybaha DNA-da oo gaaban sida 30bp iyo inta u weyn sida 10kb waa la soo celin karaa.

◮Waxtarka soo kabashada sare:Waxtarka ugu sarreeya ee soo kabashada wuxuu gaari karaa in ka badan 80%.

◮Nadaamka yar-yar:ugu yaraan 30μl xal elution waxaa loo isticmaali karaa elution, kaas oo si wax ku ool ah u kordhin kara fiirsashada ee jajabkii DNA soo kabsaday.

◮Xawaaraha dheereeya:Si fudud in loo shaqeeyo, soo kabashada jajabka DNA-da waxaa lagu dhameyn karaa 15 daqiiqo gudahood.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareQaybaha DNA-da ee la helay waa nadiif sare, kuwaas oo la kulmi kara tijaabooyin kala duwan oo xiga.

weydiin tafatiran
EndoFree Maxi Plasmid Kit (Qalabka Spin)

Nidaamka soo saarista oo dhan wuxuu qaadanayaa 1 saac oo keliya, isagoo hubinaya in ku habboon oo degdeg ah hawlgalka.

weydiin tafatiran