Alaab-qeybiyeyaasha iyo Warshada |Soo-saareyaasha Alaabta Shiinaha

Qalabka tooska ah ee RT-qPCR

◮Wixii RNA toos ah-waqtiga-dhabta ah u kordhinta ururinta suufka iyada oo aan lahayn hababka sifaynta RNA.Qalabkani waxa uu ku dhamaystirayaa wareegyada qRT-PCR hal saac gudaheed.Isku darka enzyme waa isku dhafka la hagaajiyay ee Reverse Transcriptase, Hot-Start Taq DNA polymerase, horjoogaha RNase.Reaction Buffer wuxuu ka kooban yahay dhammaan qaybaha loo baahan yahay, oo ay ku jiraan qaybaha kaydinta la hagaajiyay, Mg²⁺, dUTP, iyo dNTPs.

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Xirmada Go'doominta DNA ee FFPE Qalabka Sifaynta DNA ee Ka Soo Bixinta Tusaalooyinka FFPE

Si dhakhso leh uga nadiifi DNA-ga genomic-ga tayada sare leh unugyo ku-xidhan paraffin-ka sida qaybaha paraffin iyo blocks paraffin.

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

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Qalabka Nadiifinta PCR Nidaamka Nadiifinta PCR ee DNA-da saafiga ah

Si dhakhso leh u nadiifi oo u hel jajabyada DNA-da tayada sare leh ee nidaamka PCR;Haddii aad rabto inaad kala tagto oo aad hesho jajab DNA oo gaar ah, fadlan dooro xirmada soo kabashada jel.

◮ Soo kabashada DNA-da oo ballaadhan: Qaybaha DNA-da oo gaaban sida 60bp iyo inta 10kb ah waa la soo ceshan karaa.

◮ Waxtarka soo kabashada sareWaxtarka soo kabashada guud ahaan waa in ka badan 80%, kan ugu sarreeyana wuxuu gaari karaa in ka badan 95%.

Xawaaraha dheereeya:Fudud in la shaqeeyo, soo kabashada jajabka DNA-da waxaa lagu dhameyn karaa 15 daqiiqo gudahood iyo dimer-ka si toos ah ayaa loo saari karaa iyada oo aan dib u soo kabashada jel.

◮ SafeefSoo saarista reagent organic looma baahna.

◮ Tayo sare: Qaybaha DNA-da ee la helay waa nadiif sare, kuwaas oo la kulmi kara tijaabooyin kala duwan oo xiga.

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Tusaalaha Wakiilka Siideynta

◮ fududahay in lagu shaqeeyo:muunad lysis inta muunad ururin

◮ Degdeg ah oo ku habboon:Looma baahna in la soo saaro aashitada nucleic, oo ku habboon ogaanshaha baaxadda weyn

◮ Baaxadda codsiga:PCR si toos ah ka dib ururinta muunad

◮Amaan oo la aamini karo:Fayraska degdega ah u hawlgeli, dammaanad qaadka badbaadada ilaa xadka ugu weyn

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RT-PCR Easyᵀᴹ I (Hal Tallaabo)

◮Xirmada hal-tallaabo ah waxay awood u siinaysaa qoraal-celinta iyo PCR in lagu sameeyo isla tuubo isku mid ah.Waxa kaliya oo ay u baahan tahay in lagu daro template RNA, asal PCR gaar ah iyo ddH-RNase-free₂O.

◮Falanqaynta tirada waqtiga-dhabta ah ee RNA si dhakhso ah oo sax ah ayaa loo samayn karaa.

◮Qalabku waxa uu isticmaalaa reagent transcription u gaar ah Foregene iyo Foregene HotStar Taq DNA Polymerase oo ay weheliso nidaam falcelin gaar ah si si wax ku ool ah loo wanaajiyo waxtarka xoojinta iyo gaar ahaaneed ee falcelinta.

◮Habka falcelinta ee la wanaajiyey ayaa ka dhigaysa fal-celintu inay yeelato dareen-helid sare, degganaansho kulayleed oo xooggan, iyo dulqaad wanaagsan.

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RT-PCR Easyᵀᴹ II (Laba Talaabo)

◮Falanqaynta tirada waqtiga-dhabta ah ee RNA si dhakhso ah oo sax ah ayaa loo samayn karaa.

◮Qalabku waxa uu isticmaalaa reagent transcription u gaar ah Foregene iyo Foregene HotStar Taq DNA Polymerase oo ay weheliso nidaam falcelin gaar ah si si wax ku ool ah loo wanaajiyo waxtarka xoojinta iyo gaar ahaaneed ee falcelinta.

◮ Habka falcelinta ee la wanaajiyey ayaa ka dhigaysa fal-celintu inay yeelato dareen-helid sare, degganaansho kulayleed oo xooggan, iyo dulqaad wanaagsan.

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Mouse Tail DNA Mini Kit Xirmooyinka Soo saarista DNA ee Genomic ee Dabada Mouse

Qalabka ugu dhaqsaha badan adduunka ee lagu soo saaro DNA-da dabada jiirka, kaas oo 1 saac gudaheed ka soo saari kara DNA-ga genomic-ga ah ee tayada sare leh.

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

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Xirmooyinka Go'doominta DNA-da bakteeriyada Bakteeriyada Genomic DNA Xirmooyinka Sifaynta

Si dhakhso leh uga nadiifi DNA-ga tayada sare leh ee bakteeriyada ee marxaladda koritaanka logarithmic.

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

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CCND1/IGH Dual Fusion Baaritaanka Labada Midab

Marka loo eego mabda'a DNA-ga lammaane kaabaya, baaritaanka CCND1 orange-cas iyo baaritaanka cagaarka IGH ayaa loo isticmaalay in lagu dhex milmo isku xigxiga bartilmaameedka DNA ee xudunta, iyo xogta gobolka hidda-wadaha ee nukleus ayaa lagu arkay oo lagu falanqeeyay iyada oo hoos timaada mikroskoob fluorescence ah.

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RB1/1q21 Baadhitaanka Midabka Dual

Fluorescence in situ hybridization (FISH) waxay ku salaysan tahay mabda'a lammaanaynta dhammaystirka ah ee saldhigyada DNA-da, iyo aragga calaamadaha isku-dhafka ee baaritaanka DNA-da ee fluorescence-ka leh ee taxanaha bartilmaameedka DNA-da ee nukleus unugga hoos yimaada mikroskoob ee fluorescence.

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HER2/CSP17 Baadhitaanka Midabka Dual

Fluorescence in situ hybridization (FISH) waxay ku salaysan tahay mabda'a lammaanaynta dhammaystirka ah ee saldhigyada DNA-da, iyo aragga calaamadaha isku-dhafka ee baaritaanka DNA-da ee fluorescence-ka leh ee taxanaha bartilmaameedka DNA-da ee nukleus unugga hoos yimaada mikroskoob ee fluorescence.

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ETV6 Dual Color Break Apart Probe

Marka loo eego mabda'a isku-dhafan ee saldhigga DNA-ga, baaritaanka ETV6-oranji-cas iyo baaritaanka cagaaran ETV6 ayaa loo adeegsaday isku-dhafka isku-dhafka bartilmaameedka DNA-da ee nukleus-ka, iyo xogta gobolka hidda-wadaha ee nukleus-ka ayaa lagu arkay oo lagu lafa-guray hoostiisa mikroskoob fluorescence ah.

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