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RT-PCR Easyᵀᴹ I (Hal Tallaabo)
◮Xirmada hal-tallaabo ah waxay awood u siinaysaa qoraal-celinta iyo PCR in lagu sameeyo isla tuubo isku mid ah.Waxa kaliya oo ay u baahan tahay in lagu daro template RNA, asal PCR gaar ah iyo ddH-RNase-free2O.
◮Falanqaynta tirada waqtiga-dhabta ah ee RNA si dhakhso ah oo sax ah ayaa loo samayn karaa.
◮Qalabku waxa uu isticmaalaa reagent transcription u gaar ah Foregene iyo Foregene HotStar Taq DNA Polymerase oo ay weheliso nidaam falcelin gaar ah si si wax ku ool ah loo wanaajiyo waxtarka xoojinta iyo gaar ahaaneed ee falcelinta.
◮Habka falcelinta ee la wanaajiyey ayaa ka dhigaysa fal-celintu inay yeelato dareen-helid sare, degganaansho kulayleed oo xooggan, iyo dulqaad wanaagsan.
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Mouse Tail DNA Mini Kit Xirmooyinka Soo saarista DNA ee Genomic ee Dabada Mouse
Qalabka ugu dhaqsaha badan adduunka ee lagu soo saaro DNA-da dabada jiirka, kaas oo 1 saac gudaheed ka soo saari kara DNA-ga genomic-ga ah ee tayada sare leh.
◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.
◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.
◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.
◮Badbaadada:Looma baahna soo saarista reagent organic.
◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.
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Xirmooyinka Go'doominta DNA-da bakteeriyada Bakteeriyada Genomic DNA Xirmooyinka Sifaynta
Si dhakhso leh uga nadiifi DNA-ga tayada sare leh ee bakteeriyada ee marxaladda koritaanka logarithmic.
◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.
◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.
◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.
◮Badbaadada:Looma baahna soo saarista reagent organic.
◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.
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ATM/CSP11 Baadhitaanka Midabka Dual
Marka loo eego mabda'a isku-dhafan ee saldhigyada DNA-da, baaritaanka ATM orange-ka iyo baaritaanka cagaarka ee CSP11 ayaa loo adeegsaday isku-dheellitirka taxanaha bartilmaameedka DNA ee xudunta, iyo heerka xogta heerka hidda-wadaha ee nukleus ayaa la arkay oo lagu falanqeeyay iyada oo la raacayo mikroskoob fluorescence.
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ERG1/TERT Baadhitaanka Midabka Dual
Fluorescence in situ hybridization (FISH) waxay ku salaysan tahay mabda'a lammaanaynta dhammaystirka ah ee saldhigyada DNA-da, iyo aragga calaamadaha isku-dhafka ee baaritaanka DNA-da ee fluorescence-ka leh ee taxanaha bartilmaameedka DNA-da ee nukleus unugga hoos yimaada mikroskoob ee fluorescence.
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Qalabka Soo saarista DNA/RNA
- Habka go'doominta oo dhan waa badbaado oo habboon
- Unugga la soo saaray DNA-da xorta ah wuxuu leeyahay waxsoosaar sare, nadiif sare iyo tayo la isku halayn karo
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20q12/20q13.33 Baadhitaanka Midabka Dual
Marka loo eego mabda'a isku-dhafan ee saldhigyada DNA-da, 20q12 (D20S108) baaritaanka orange iyo 20q33.3 baaritaanka cagaarka ah ayaa loo isticmaalay in lagu dhex daro taxanaha bartilmaameedka DNA ee nukleus, iyo macluumaadka gobolka hidda-wadaha ee nukleus ayaa la arkay oo lagu falanqeeyay hoostiisa mikroskoob ee fluorescence.
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p53/D13S319 Baadhitaanka Midabka Dual
Fluorescence in situ hybridization (FISH) waxay ku salaysan tahay mabda'a lammaanaynta dhammaystirka ah ee saldhigyada DNA-da, iyo aragga calaamadaha isku-dhafka ee baaritaanka DNA-da ee fluorescence-ka leh ee taxanaha bartilmaameedka DNA-da ee nukleus unugga hoos yimaada mikroskoob ee fluorescence.
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EndoFree Maxi Plasmid Kit (Qalabka Spin)
Nidaamka soo saarista oo dhan wuxuu qaadanayaa 1 saac oo keliya, isagoo hubinaya in ku habboon oo degdeg ah hawlgalka.
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MALT1 Baadhitaanka Kala-baxa Labada Midab
Marka loo eego mabda'a saldhigga DNA-ga lammaane kaabaya, baaritaanka MALT1 orange-cas iyo baaritaanka cagaarka MALT1 ayaa loo adeegsaday in lagu dhex miiro taxanaha bartilmaameedka DNA ee xudunta, iyo macluumaadka gobolka hidda-wadaha ee nukleus-ka ayaa la arkay oo lagu falanqeeyay iyada oo la raacayo mikroskoob fluorescence ah.
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D7S522/CSP7 Baadhitaanka Midabka Dual
Fluorescence in situ hybridization (FISH) waxay ku salaysan tahay mabda'a lammaanaynta dhammaystirka ah ee saldhigyada DNA-da, iyo aragga calaamadaha isku-dhafka ee baaritaanka DNA-da ee fluorescence-ka leh ee taxanaha bartilmaameedka DNA-da ee nukleus unugga hoos yimaada mikroskoob ee fluorescence.
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DAPI Counterstain
Qaybta ugu muhiimsan ee xalinta dib-u-midabaynta DAPI waa 4-phenylindole, kaas oo geli kara xuubka unugyada oo si adag ugu xidhi kara DNA-da.
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