Alaab-qeybiyeyaasha iyo Warshada Molecular Biology reagent |Soo-saareyaasha noolaha noolaha ee Shiinaha Molecular Biology

Biyolojiye Molecular

Taxanaha go'doominta RNA

Taxanaha go'doominta DNA

Taxanaha tooska ah ee RT-qPCR

Taxanaha PCR ee tooska ah ee dhiiga

Taxanaha PCR ee tooska ah ee dhirta

Taxanaha PCR tooska ah ee xayawaanka

PCR/RT-PCR taxane

IVD alaabta ceeriin

Genomic DNA Micro Kit Micro Genomic DNA Mini Kit

Si dhakhso leh u nadiifi muunado yaryar si aad u hesho DNA-ga genomic oo tayo sare leh.

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

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Nidaamka Aqoonsiga DNA ee Foregene Y Plus (aan-soo-saarid la'aan)

ForegeneHabka aqoonsiga DNA-da Y Plus waxay isticmaashaa tignoolajiyada calaamadaynta fluorescent lix midab leh si ay u kordhiso 20 Y koromosoom core loci, 15 loci doorbiday, 6 beddelka loci iyo 3 Y indel loci hal mar.

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Xirmada Go'doominta DNA-da ee aan unugga lahayn

Ka soocida cfDNA ee shaybaarada balaasmaha

Lambarka buugga TQ01BT0050, TQ01BT0100

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Nidaamka Aqoonsiga DNA ee Foregene 25A-C (Kiiska)

Nidaamka Aqoonsiga DNA-da ee Foregene 25A-C waxay isticmaashaa tignoolajiyada calaamadaynta fluorescence lix midab leh si ay u xoojiso 23 autosomal STR loci, hal Amel loci, hal Yindel loci iyo laba goob xakamaynta tayada gudaha markiiba si loo kordhiyo habab DNA-da oo laga soo saaro oo laga nadiifiyo muunado kiis.

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Nidaamka Aqoonsiga DNA ee Foregene 20A ( Soo saarista DNA-da bilaashka ah)

Nidaamka aqoonsiga DNA-da ee Foregene 20A wuxuu isticmaalaa tignoolajiyada calaamadaynta fluorescent shan midab leh si ay isku mar u xoojiso 19 STR loci iyo 1 loci galmo oo hal tuubo ah.Marka la daboolayo 13 CODIS core loci, 20 core loci ayaa buuxinaya shuruudaha Wasaaradda Amniga Dadweynaha ee xirmada STR ee iswada.Waa gacanta midig ee dhismaha xogta DNA-da ee dembi-baarista iyo aqoonsiga qaraabada garsoorka.

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Nidaamka Aqoonsiga DNA ee Foregene 27Y

ForegeneNidaamka Aqoonsiga DNA 27Y wuxuu isticmaalaa tignoolajiyada calaamadaynta fluorescent ee lix midab leh si uu u kordhiyo 27 Y-STR loci hal mar.Waxaa ka mid ah: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS448, DYS533, DYFY387 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.

Qalabku wuxuu leeyahay awood ka hortag ah oo wanaagsan, wuxuuna si toos ah u kordhin karaa waraaqaha shaandhada, kaarka FTA, suufka suufka, kaarka candhuufta, suufka afka, iyo sidoo kale waxaa loo isticmaali karaa in laga soo saaro oo lagu nadiifiyo qaababka DNA-da ee alaabta kiiska.

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Tooska RT-qPCR Kit II

◮Qalabka tooska ah ee RT-qPCR, iyadoo la adeegsanayo Foreasy Reverse Transcriptase iyo Foreasy HS Taq DNA Polymerase ee uu soo saaray Foregene, oo leh falcelin gaar ah, ayaa ka dhigaysa xirmadan iska caabin xooggan iyo waafaqid, waxayna si toos ah u tijaabin kartaa falcelinta adeegsiga nidaamka Foregene Lysis qaab qaab ahaan.Qalabkani waxa uu dalban karaa xirmada ogaanshaha aashitada nucleic ee COVID-19 iyo horumarinta xirmada ogaanshaha asiidhka kale ee bakteeriyada.

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Tooska RT-qPCR Kit III

◮Qalabka tooska ah ee RT-qPCR, iyadoo la adeegsanayo Foreasy Reverse Transcriptase iyo Foreasy HS Taq DNA Polymerase ee uu soo saaray Foregene, oo leh falcelin gaar ah, ayaa ka dhigaysa xirmadan iska caabin xooggan iyo waafaqid, waxayna si toos ah u tijaabin kartaa falcelinta adeegsiga nidaamka Foregene Lysis qaab qaab ahaan.Qalabkani waxa uu dalban karaa xirmada ogaanshaha aashitada nucleic ee COVID-19 iyo horumarinta xirmada ogaanshaha asiidhka kale ee bakteeriyada.

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Qalabka tooska ah ee RT-qPCR

◮ Qalabka tooska ah ee RT-qPCR, iyadoo la adeegsanayo Foreasy Reverse Transcriptase iyo Foreasy HS Taq DNA Polymerase ee uu soo saaray Foregene, oo leh falcelin gaar ah, ayaa ka dhigaysa xirmadan iska caabin xooggan iyo waafaqid, waxayna si toos ah u tijaabin kartaa falcelinta adeegsiga nidaamka Foregene Lysis qaab qaab ahaan.Qalabkani waxa uu dalban karaa xirmada ogaanshaha aashitada nucleic ee COVID-19 iyo horumarinta xirmada ogaanshaha asiidhka kale ee bakteeriyada.

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Xirmada Go'doominta MiRNA ee Xayawaanka Soo saarista iyo Xirmooyinka Sifaynta ee Xoolaha

Si dhakhso leh oo hufan uga soo saar jajabyada RNA yaryar ee 20-200nt miRNA, siRNA, snRNA ee unugyada iyo unugyada kala duwan ee xayawaanka.

◆Habka oo dhan waxaa lagu maamulaa heerkulka qolka (15-25°C), iyada oo aan lahayn qubeys baraf ah iyo heerkul hooseeya.

◆Xidhmada dhamaystiran ee RNase-Free, looma baahna in laga walwalo xaalufka RNA.

◆Tiirka Nadiifinta DNA-da ayaa si gaar ah u xidha DNA-da, si uu xidhmoku uga saaro wasakhaynta DNA-da iyada oo aan lagu darin DNA-da dheeraad ah.

◆Wax-soosaarka RNA sare: Tiirka-RNA-kaliya iyo qaacido gaar ah ayaa si hufan u nadiifin kara RNA.

◆Xawaaraha dheereeya: Qalliinku waa sahlan yahay waxaana lagu dhamayn karaa 30 daqiiqo gudahood.

◆Badbaadada: soo saarista reagents organic looma baahna.

◆Tayo sare leh: Qaybaha RNA ee la soo saaray waa nadiif sare, ka madax banaan borotiin iyo wasakh kale, waxayna buuxin karaan shuruudaha hoose.

◆Codsiyada tijaabada ah ee kala duwan

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Xirmada Go'doominta DNA ee Biyaha Xirmada Soosaarida iyo Sifaynta DNA ee Biyaha

Si deg deg ah uga nadiifi DNA-ga genomic-ga tayada sare leh ee ka yimaadda microorganisms ee muunado biyo oo kala duwan.

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

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Abuurka Dhirta (Polysaccharide Polyphenol qani, weyn iyo dhexdhexaad ah) PCR Tooska ah Kit II-UNG (aan lahayn Qalabka Muunalada)

Si toos ah u isticmaal polysaccharide polyphenol iniin lysate qaab template ah kor u qaadida PCR, iyada oo aan la soo saarin DNA gaar ah, degdeg iyo dareen sare.Nidaamka ka hortagga wasakheynta ee UNG ayaa lagu daray si looga hortago waxyaabo been abuur ah.

◮Looma baahna nadiifin waqti-qaadasho iyo qaali ah.

◮Dalabka muunadku wuu yar yahay, kaliya qaado hal abuur.

◮Looma baahna daaweyn gaar ah sida shiidi iyo burburin, qalliinkuna waa sahlan yahay.

◮Nidaamka PCR ee la hagaajiyay wuxuu awood u siinayaa PCR inuu yeesho gaar gaar ah iyo dulqaad adag oo loogu talagalay horjoogayaasha falcelinta PCR.

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