Alaab-qeybiyeyaasha iyo Warshada Molecular Biology reagent |Soo-saareyaasha noolaha noolaha ee Shiinaha Molecular Biology

Biyolojiye Molecular

Taxanaha go'doominta RNA

Taxanaha go'doominta DNA

Taxanaha tooska ah ee RT-qPCR

Taxanaha PCR ee tooska ah ee dhiiga

Taxanaha PCR ee tooska ah ee dhirta

Taxanaha PCR tooska ah ee xayawaanka

PCR/RT-PCR taxane

IVD alaabta ceeriin

Qalabka yar ee DNA-ga

Si dhakhso leh uga nadiifi DNA-ga genomic ee tayada sare leh dhiiga xinjirowga lidka ku ah (<1ml).

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

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Xirmooyinka Yaryar ee Plasmid-ka Guud ee Plasmid-ka Soo saaridda Miniprep Mini Kits

Si dhakhso leh uga nadiifi DNA-da plasmid ee tayada sare leh bakteeriyada la beddelay ee tijaabooyinka bayoolojiga joogtada ah sida isbeddelka iyo dheefshiidka ensaymka.

◮Ka saar RNA adigoon ku darin RNase

◮Ku habboon-centrifugation-ka waxaa lagu sameeyaa heerkulka qolka

◮Degdeg ah-qaliinka waxa lagu dhamayn karaa 20 daqiiqo

◮Ammaan- lama isticmaalo reagent organic

◮daahirnimo sare-OD260/280≈1.7-1.9

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RT Easyᵀᴹ I Master Premix-ka ugu horreeya ee cDNA-yada

-Nidaamka dib-u-dejinta oo hufan, kaliya waxay qaadataa 25 daqiiqo in la dhammaystiro isku-darka cDNA-ga ugu horreeya.

- Nidaamka qoraalka dib u celinta dareenka sare, jaantusyada heerka pg sidoo kale waxay heli karaan cDNA tayo sare leh.

- Nidaamka qoraalka dib-u-celinta wuxuu leeyahay xasillooni heerkul sare ah, heerkulka falcelinta nidaamku wuxuu noqon karaa mid sarreeya 50 ℃,waxayna leedahay wax qabad wanaagsan oo qoraal ah.

- Xataa muunadaha RNA ee aan nadiifka ahayn (khamriga ilaa 45%, cusbada guanidine ilaa 750mM) ayaa lagu soo rogi karaa falcelin qoraal ah.

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RT Easy II (oo leh gDNase) Master Premix oo loogu talagalay isku-dhafka cDNA-ga ugu horreeya ee PCR ee leh gDNase

-Awood waxtar leh oo looga saarayo gDNA, taas oo ka saari karta gDNA qaab-dhismeedka 2 daqiiqo gudahood.

-Nidaamka dib-u-dejinta oo hufan, waxay qaadataa 15 daqiiqo oo keliya in la dhammaystiro isku-darka cDNA-ga ugu horreeya.

Hababka isku dhafan: qaab-dhismeedka ka kooban GC-ga sare iyo qaab-dhismeedka sare ee adag ayaa sidoo kale lagu beddeli karaa hufnaan sare.

-Nidaamka qoraalka dib u celinta dareenka sare, jaantusyada heerka pg sidoo kale waxay heli karaan cDNA tayo sare leh.

-Nidaamka qoraalka dib u celinta ayaa leh xasilooni kulayl sare leh, heerkulka falcelinta ugu fiican waa 42℃, oo wali waxay leedahay wax qabad wanaagsan oo qoraal ah oo 50 ah℃.

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Qalabka PCR ee wakhtiga dhabta ah Easyᵀᴹ-SYBR Cagaaran I xirmada

Fudud-2X PCR Isku-dhafan si loo yareeyo khaladka tijaabada iyo wakhtiga hawlgalka

Bakhaar gaar ah oo la hagaajiyay iyo bilowga kulul ee Taq enzyme waxay ka hortagi kartaa kordhinta aan gaarka ahayn iyo samaynta dimer ka

Dareenka sare-wuxuu ogaan karaa nuqullo hoose oo template ah

Kala duwanaansho wanaagsan - oo ku habboon inta badan qalabka PCR ee tirada dhabta ah

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Xirmooyinka Go'doominta RNA Wadarta Xayawaanada

Si degdeg ah oo hufan uga soo saar wadarta guud ee RNA nadiif-sare iyo tayo sare leh oo laga soo saaray unugyo xayawaanno kala duwan.

Looma baahna in laga walwalo xaalufka RNA.Nidaamka oo dhan waa RNase-free

Si wax ku ool ah uga saar DNA adoo isticmaalaya Tiirka Nadiifinta DNA

Ka saar DNA adigoon ku darin DNA-ga

Fudud - dhammaan hawlgallada waxaa lagu dhammeeyaa heerkulka qolka

Dhaqso-qalliinka waxa lagu dhamayn karaa 30 daqiiqo gudahood

Badbaadsan — lama isticmaalin reageent organic

Nadaafadda sare-OD260/280≈1.8-2.1

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Qalabka Go'doominta Unugga ee RNA Wadarta Xirmooyinka Go'doominta ee unugga

Wadarta guud ee RNA oo si aad ah loo sifeeyay oo tayo sare leh ayaa laga heli karaa unugyo dhaqan oo kala duwan 11 daqiiqo gudaheed.

RNase-free

Ku shaqeeya heerkulka qolka (15-25 ℃)

Iyada oo leh Tiirka Nadiifinta DNA

Wax soo saarka RNA sare

Degdeg: Ku dhamee soo saarista 11 daqiiqo gudahood

Badbaadada: Ma jiro kiimiko dabiici ah

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Qalabka Midi-ga ee DNA-da Dhiiga

Si dhakhso leh uga nadiifi DNA-ga genomic ee tayada sare leh dhiiga xinjirowga lidka ku ah (1-5ml).

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

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Qalabka Go'doominta ee Kaarka FTA ee Kaarka DNA-ga Soosaarida ama Xirmada Sifaynta

Si dhakhso leh uga nadiifi DNA-ga genomiga tayada sare leh ee swab buccal/Samballada Kaarka FTA

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

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Nidaamka Aqoonsiga DNA ee Foregene 27Y

ForegeneNidaamka Aqoonsiga DNA 27Y wuxuu isticmaalaa tignoolajiyada calaamadaynta fluorescent ee lix midab leh si uu u kordhiyo 27 Y-STR loci hal mar.Waxaa ka mid ah: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS448, DYS533, DYFY387 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.

Qalabku wuxuu leeyahay awood ka hortag ah oo wanaagsan, wuxuuna si toos ah u kordhin karaa waraaqaha shaandhada, kaarka FTA, suufka suufka, kaarka candhuufta, suufka afka, iyo sidoo kale waxaa loo isticmaali karaa in laga soo saaro oo lagu nadiifiyo qaababka DNA-da ee alaabta kiiska.

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Nidaamka Aqoonsiga DNA ee Foregene 38Y-C (Kiiska)

ForegeneHabka aqoonsiga DNA 38Y-C waxay isticmaashaa tignoolajiyada calaamadaynta fluorescent-ka midabka leh si ay u xoojiso 38 Y koromosoomyada STR loci, 3 Y-Indel lociiyolaba kontorool tayada gudaha hal marsi loo kordhiyo shaybaarada Kiis laga soo saaray qaabab DNA oo la safeeyey.

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Nidaamka Aqoonsiga DNA ee Foregene 30A (aan-soosaar la'aan)

FOREGENE Nidaamka aqoonsiga DNA 30A wuxuu isticmaalaa tignoolajiyada calaamadaynta fluorescent-ka midabka lehweynayn 29autosomalSTR meesha, midAmel locus iyo hal Yindel locus hal mar, oo si toos ah u xoojin kara warqad shaandhaysa, FTAkaararka, suufka suufka, kaararka candhuufta,iyo msuuf ka baxsan.

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