Taxanaha tooska ah ee RT-qPCR

PCR toos ah

Waa maxay Direct PCR

PCR tooska ah waa habka DNA-ga si toos ah loogu kordhiyo dhiigga, unugyada xayawaanka, dabada jiirka, unugga, dhegta jiirka, kalluunka zebra, ukunta kalluunka, caleemaha dhirta, abuurka ama muunada kale ee unugyada noolaha ee asalka ah iyada oo aan la samaynin go'doomin DNA iyo tillaabooyinka nadiifinta.Farsamada tooska ah ee PCR waxay si weyn u dhimi kartaa wakhtiga tijaabada, iyo qiimaha genotyping iyo mashaariicda tirada badan.Waxa kale oo ay bixisaa ikhtiyaar ka wanaagsan marka ay la kulmaan caqabadaha kor u qaadida tiro aad u yar oo muunad ah halkaas oo tallaabada sifeyntu ay u horseedi karto muunad lumi.

FOREGENE's PCR Mix waxay leedahay iska caabin adag oo walbahaarka, sababtoo ah FOREGENE xoogga patented Taq enzyme (patent idman: ZL20161034512.0 (Shiinaha), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)Its enzyme Taq waxay isticmaashaa tignoolajiyada dib-u-habaynta DNA si ay dib ugu soo celiso qaab dhismeedka DNA-da ee isku xidhka borotiinka nucleic acid ee ku xidha borotiinka kulaylka u adkaysta ee Taq DNA polymerase si loo dhiso qaab DNA ah oo xiriir la leh.Taq-kuleyl oo la hagaajiyay oo xajiya hawlo kala duwan ee polymerase DNA-ga asalka ah. Tan Taq enzyme waxay ku xidhi kartaa qaabaynta DNA waxayna bilaabi kartaa isku-darka DNA-ga qaab-fiirsasho hooseeya iyo deegaan adag.

Tusaale ahaan, way fududahay in lagu daro unuggaaga, geedka (caleemaha yar yar, xididka ama abuur) ama muunadda xayawaanka ee kaydinta, oo lagu daro isku darka sayidkiisa gaarka ah oo leh qalab gaar ah oo ku jira tuubooyinka PCR oo ku dhex maro wareegtada kulaylka.Hufnaanta kitku waxay ku habboon tahay falanqaynta muunad baaxad weyn halkaas oo wakhtiga badbaadintu aad muhiim ugu yahay.

Nidaamka falcelinta PCR ee ka kooban UNG ensaymka iyo dUTP ayaa lagu doortay iyadoo loo eegayo shuruudaha tijaabada, taas oo si wax ku ool ah uga fogaan karta wasakhowga ay sababaan alaabada kor u qaadida PCR.

Faa'iidooyinkaDPCR-ga

		PCR toos ah	Habka dhaqameed (Nadiifinta RNA+ PCR Caadiga ah)
Isticmaalka alaabta	Isticmaalka nudaha	In ka yar	Wax badan
Diyaarinta qaab-dhismeedka	Nooca walxaha	Wax shiiditaan looma baahna	Muunada shiida
	Hawlgalka	Nooca hal-tuubo, 10min	qalliin adag, 1h
	Flux	1-96	1-24
falcelinta PCR	habka falcelinta	Isku dhafka 2× PCR ee la hagaajiyay	Dntp, MgCl₂,10× PCR Buffer,Taq enzyme,

Acodsi

01 Aqoonsiga jeermiska cudurada keena

02 Aqoonsiga hidde ahaan wax laga beddelay, genotyping

03 Multiplex PCR / SNP ogaanshaha / PCR-RFLP

04Taxane/cloning

Taxanaha Alaabta

Taxane	Magaca Alaabta	Tilmaamaha	Lambarka buugaagta	Xaaladaha Kaydinta
Taxanaha tooska ah ee RT-qPCR	Degdega fudud^TMQalabka Tooska ah ee Unugyada RT-qPCR-SYBR Cagaaran I	200T	DRT-01011	Qaybta I 4℃, Qaybta II -20℃
Taxanaha tooska ah ee RT-qPCR		1000T	DRT-01012	Qaybta I 4℃, Qaybta II -20℃

Taxane	Magaca Alaabta	Tilmaamaha	Lambarka buugaagta	Xaaladaha Kaydinta
Taxanaha PCR Tooska ah ee Dhiiga	Dhiiga SuperDirect^TMQalabka PCR (EDTA)	200 × 20μl rxns	TP-04111	-20℃
		2000 × 20μl rxns	TP-04113
	Dhiiga SuperDirect^TMQalabka PCR (EDTA) -UNG	200 × 20μl rxns	TP-04121	-20℃
		2000 × 20μl rxns	TP-04123
	Dhiiga SuperDirect^TMQalabka PCR (Heparin)	200 × 20μl rxns	TP-04211	-20℃
		2000 × 20μl rxns	TP-04213
	Dhiiga SuperDirect^TMQalabka PCR (Heparin) -UNG	200 × 20μl rxns	TP-04221	-20℃
		2000 × 20μl rxns	TP-04223

Seri	Magaca Alaabta	Tilmaamaha	Lambarka buugaagta	Xaaladaha Kaydinta
Taxanaha PCR Tooska ah ee Animal	Qalabka PCR ee Unugyada Tooska ah (65℃ lysis)	200 × 20μl rxns	TP-01111	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-01113
	Qalabka PCR ee Unugyada Tooska ah (65℃ lysis) -UNG	200 × 20μl rxns	TP-01121	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-01123
	Mouse Tail SuperDirect^TMQalabka PCR (Qalabka heerkulka qolka)	200 × 20μl rxns	TP-01311	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-01313
	Qalabka Dabada Tooska ah ee Mouse Tail PCR (65℃ lysis)	200 × 20μl rxns	TP-01331	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-01333
	Mouse Tail Direct PCR Kit (65℃ lysis) - UNG	200 × 20μl rxns	TP-01341	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-01343
	Qalabka PCR ee tooska ah ee Zebra Fish (65℃ lysis)	200 × 20μl rxns	TP-01411	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-01413
	Zebra Fish Kit Direct PCR (65℃ lysis) - UNG	200 × 20μl rxns	TP-01421	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-01423

Taxane	Magaca Alaabta	Tilmaamaha	Lambarka buugaagta	Xaaladaha Kaydinta
Plant Direct PCR Taxanaha	Qalabka PCR ee tooska ah ee caleenta	200 × 20μl rxns	TP-02111	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-02113
	Qalabka Caleenta Tooska ah ee PCR -UNG	200 × 20μl rxns	TP-02121	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-02123
	Qalabka Caleenta Tooska ah ee PCR Plus (Polysaccharide polyphenol dhirta)	200 × 20μl rxns	TP-02131	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-02133
	Qalabka Caleenta Tooska ah ee PCR Plus -UNG (Polysaccharide polyphenol dhirta)	200 × 20μl rxns	TP-02141	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-02143
	Abuurka Dhirta ee tooska ah ee PCR Kit I	200 × 20μl rxns	TP-03111	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-03113
	Qalab Dhirta oo toos ah PCR Kit I-UNG	200 × 20μl rxns	TP-03121	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-03123
	Abuurka dhirta tooska ah PCR Kit II	200 × 20μl rxns	TP-03131	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-03133
	Abuurka Dhirta Qalabka PCR ee tooska ah II -UNG	200 × 20μl rxns	TP-03141	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-03143
	Seeed Plant Direct PCR Plus Kit I (Polysaccharide polyphenol warshad)	200 × 20μl rxns	TP-03151	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-03153
	Qalab Dhirta Tooska ah PCR Plus Kit I - UNG (Warshad polyphenol polysaccharide)	200 × 20μl rxns	TP-03161	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-03163
	Abuurka Dhirta Tooska ah PCR Plus Kit II	200 × 20μl rxns	TP-03171	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-03173
	Farsamada Iniinta Tooska ah PCR Plus Kit II - UNG (Warshad polyphenol polysaccharide)	200 × 20μl rxns	TP-03181	Qaybta I 4℃, Qaybta II -20℃
		2000 × 20μl rxns	TP-03183